We narrowed to 3,637 results for: golden gate

1. pKG1975_pGEEC506_pShip-hPGK-mRuby2-WPRE

   Plasmid

   #239712

   Purpose

   Plasmid expressing mRuby2 with the hPGK promoter and WPRE 3' UTR

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKG2021_pGEEC507_pShip-UbC-mRuby2-bGH

   Plasmid

   #239713

   Purpose

   Plasmid expressing mRuby2 with the UbC promoter and bGH 3' UTR

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKG2022_pGEEC508_pShip-UbC-mRuby2-SV40

   Plasmid

   #239714

   Purpose

   Plasmid expressing mRuby2 with the UbC promoter and SV40 3' UTR

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKG1367_pPV1-hPGK

   Plasmid

   #239691

   Purpose

   BsaI Golden Gate assembly plasmid encoding the hPGK promoter

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   hPGK promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Removal of BsaI cut sites

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKG0743_pPV1-ZF43x6-C

   Plasmid

   #239693

   Purpose

   BsaI Golden Gate assembly plasmid encoding the COMET ZF43x6-C promoter

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   ZF43x6-C promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKG3657_pPV1-ZF10.BSx8

   Plasmid

   #239694

   Purpose

   BsaI Golden Gate assembly plasmid encoding the synZiFTR ZF10.BSx8 promoter

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   ZF10.BSx8 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKG1055_pPV2-tagBFP

   Plasmid

   #239697

   Purpose

   BsaI Golden Gate assembly plasmid encoding the tagBFP CDS

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   tagBFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Removal of BsaI cut sites

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKG3659_pPV2-rtTA-P2A-iRFP720

   Plasmid

   #239698

   Purpose

   BsaI Golden Gate assembly plasmid encoding the rtTA-P2A-iRFP720 CDS

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   rtTA-P2A-iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKG0587_pPV3-bGH

   Plasmid

   #239701

   Purpose

   BsaI Golden Gate assembly plasmid encoding the bGH PAS

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   bGH PAS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKG0588_pPV3-SV40

    Plasmid

    #239702

    Purpose

    BsaI Golden Gate assembly plasmid encoding SV40 PAS

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    SV40 PAS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKG2019_pPV1-EF1a

    Plasmid

    #239687

    Purpose

    BsaI Golden Gate assembly plasmid encoding the EF1a promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    EF1a promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKG1988_pPV1-UbC

    Plasmid

    #239689

    Purpose

    BsaI Golden Gate assemby plasmid encoding the UbC promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    UbC promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Removal of BsaI cut sites

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid

    #239448

    Purpose

    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.

    Depositor

    Alexis Komor

    Inserts

    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    BFP: T65S, S72S, V145F, K206K, H231L

    Promoter

    EF1alpha, U6, and mU6

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHW1176

    Plasmid

    #229871

    Purpose

    gene trap RMCE plasmid with GFP-C1-tbb-2 3'UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    GFP-C1-tbb-2 3'UTR::SEC
    

    Use

    Tags

    GFP-C

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH

    Plasmid

    #235277

    Purpose

    ComMAND closed-loop circuit regulating mRuby2 with miR-FF5

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EF1a-mGL-bGH

    Plasmid

    #235299

    Purpose

    mGreenLantern expression to serve as a transfection marker, EF1a-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mGreenLantern
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH

    Plasmid

    #235282

    Purpose

    ComMAND open-loop circuit regulating mRuby2 with miR-FF4

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. U6-miR.FF4

    Plasmid

    #235256

    Purpose

    Non-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FF4 hairpin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a-mRuby2(miRE.FF5)-TS.FF6x1-bGH

    Plasmid

    #235260

    Purpose

    ComMAND open-loop circuit regulating mRuby2 with miRE-FF5

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EFS-mGL-bGH

    Plasmid

    #235255

    Purpose

    mGreenLantern expression to serve as a transfection marker, EFS-driven

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mGreenLantern
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS (human EF1a short, intronless)

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge