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pKG2440_pGEEC536_pShip-UbC-mRuby2-P2A-PuroR-bGH

Plasmid

#239732

Purpose

Plasmid expressing mRuby2 and PuroR with the UbC promoter

Depositor

Kate Galloway

Publication

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1062

Plasmid

#236186

Purpose

BsaI compatible kanamycin resistance cassette

Depositor

Jeffrey Barrick

Publication

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

Kanamycin resistance cassette

Expression

Bacterial

Promoter

CP25

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM30

Plasmid

#233502

Purpose

Level 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianus

Depositor

Vincent Martin

Publication

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

TDH3-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM31

Plasmid

#233503

Purpose

Level 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianus

Depositor

Vincent Martin

Publication

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

PGK1-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSWITCH

Plasmid

#222402

Purpose

Expression in Streptococcus pyogenes

Depositor

Anne Botteaux

Publication

Schiavolin et al Microbiol Spectr. 2024 Oct 3;12(10):e011

Type

Empty backbone

Expression

Bacterial

Available since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pKS1576

Plasmid

#239524

Purpose

Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

Depositor

John Dueber

Insert

ScVba2p-mTurquoise2-2xCrk_SH3

Expression

Yeast

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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Bo_NPTII:pATML:Gus_introns

Plasmid

#215183

Purpose

Evaluating guard cell specific promotors in Brassica

Depositor

Penny Hundleby

Publication

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

Meristem layer1

Use

Synthetic Biology

Promoter

AtML1

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRO227

Plasmid

#233835

Purpose

MoClo-YTK; ConL1/RE cassette; rtTA systems; tetO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

tetO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO236

Plasmid

#233847

Purpose

MoClo-YTK; ConL2/RE cassette; ABA systems; phlfO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

phlfO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN020

Plasmid

#233850

Purpose

MoClo-YTK; ConL2/RE cassette; Auxin systems; amtRO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

amtRO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN069

Plasmid

#233855

Purpose

MoClo-YTK; ConL2/RE cassette; NS3 systems; lexAO6-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

lexAO6-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL004

Plasmid

#233863

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL003

Plasmid

#233864

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO174

Plasmid

#233809

Purpose

MoClo-YTK; type3A; ABA systems; Phlf-NES

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Phlf-NES

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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hPGK-mRuby2-bGH

Plasmid

#235254

Purpose

ComMAND base gene regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH

Plasmid

#235294

Purpose

ComMAND closed-loop circuit regulating mRuby2 with 2 copies of microRNA target sites

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235320

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mGL(miRE.FF4)-bGH

Plasmid

#235264

Purpose

microRNA expression for dual-vector iFFL circuit

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCB036 FoMV-sgGFP

Plasmid

#234813

Purpose

To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)

Depositor

Daniel Voytas

Publication

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

gRNA targeting GFP

Expression

Plant

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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