We narrowed to 5,185 results for: golden gate
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Plasmid#234196PurposeHeterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits
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pAIP4_3KT9
Plasmid#234192PurposeHeterologous protein expression of aprataxin in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5KUW
Plasmid#234183PurposeHeterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2EYI
Plasmid#234173PurposeHeterologous protein expression of alpha-actinin-1 in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_4WDC
Plasmid#234153PurposeHeterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coliDepositorInsert4WDC
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSR1154
Plasmid#221653PurposeTetracycline sensor v3 for Staphylococcus aureusDepositorInsertssfGFP cassette
TetR cassette
UseSynthetic BiologyExpressionBacterialPromoterPXyl-TetO and PZwfAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi
Plasmid#216166PurposeExpress the gRNAs for the human FOXA1/A2/A3-CRISPRiDepositorInsertUseLentiviralTagsEGFPPromoterphU6, ph7SK, phH1, pmU6Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent
Plasmid#229800PurposeBxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1aDepositorInsertmGreenLantern; mRuby2
UseSynthetic BiologyExpressionMammalianPromoterhEF1a and CAGAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
USUV strain Vienna 2001 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228658PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Usutu virus for biochemical assayDepositorInsertUsutu virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
POWV strain LB NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228654PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assayDepositorInsertPowassan virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialMutationAlanine mutant to stop self-cleaving ..VEREEA*AL.…Available SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228653PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assayDepositorInsertMVEV portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Poliovirus 1 PV1 isolate 09_05_19 3C protease
Plasmid#228647PurposeBacterial expression of the Polio 3C proteaseDepositorInsertPolio virus 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease
Plasmid#228644PurposeBacterial expression of the D68 3C proteaseDepositorInsertEnterovirus D68 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002
Plasmid#228639PurposeBacterial expression of the Coxsackievirus 33 isoform B 3C proteaseDepositorInsertCoxsackievirus 33 isoform B 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHW1385
Plasmid#229879PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTRDepositorInsertPmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
TagsmScarlet-I-C1ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
MbCas12a_K487_GPG_GR2
Plasmid#231577PurposeEncodes a cortisol inducible variant of MbCas12aDepositorInsertMbCas12a with a GR2 insertion behind K487
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
PDCD1-DEM (P1#5)
Plasmid#227738PurposeDesigner Epigenome Modifier (DEM) designed to target the PDCD1-promoterDepositorInsertDesigner epigenome modifier targeting PDCD1-promoter
UseTALENTagsDNMT3A, DNMT3L, KRABExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LAG3-DEM (L3#1)
Plasmid#227739PurposeDesigner Epigenome Modifier (DEM) designed to target the LAG3-promoterDepositorInsertDesigner epigenome modifier targeting LAG3-promoter (Homo sapiens)
UseTALENTagsDNMT3A, DNMT3L, KRABExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330_SYCP3_nterm
Plasmid#222524PurposeCas9/sgRNA plasmid for targeting SYCP3DepositorInsertCas9, SYCP3 sgRNA (SYCP3 Synthetic, Human)
UseCRISPRAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227908PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only