We narrowed to 10,418 results for: pET

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pETDuet RagA(T21N)-RagC

Plasmid

#99644

Purpose

Bacterial expression of RagA(T21N)-RagC

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

T21N

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21b-Caspase-3(D3A,V266E)

Plasmid

#90091

Purpose

Expresses caspase D3A and V266E mutant in bacterial cells

Depositor

Clay Clark

Publication

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A V266E

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a SUMO Nup53xl 1-320

Plasmid

#85998

Purpose

bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Publication

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

SUMO

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pet16b ALIX Bro1 (1-359)

Plasmid

#80641

Purpose

Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

Depositor

Wesley Sundquist

Publication

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

ALIX Bro1, residues 1-359 (PDCD6IP Human)

Tags

His Tag

Expression

Bacterial

Mutation

Residues 1-359

Promoter

T7

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28b-LanCL2 (6xHis-LanCL2)

Plasmid

#73407

Purpose

Expresses His tagged LanCL2

Depositor

Jie Chen

Publication

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

LanCL2 (LANCL2 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pETM6-At4CL-CmCHS-MsCHI

Plasmid

#73394

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Publication

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

Plasmid

#73404

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Publication

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET22b-T7-6h-GST-α-Synuclein

Plasmid

#225224

Purpose

Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

Depositor

Urartu Seker

Publication

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

GST (GSTK1 E. coli)
α-Synuclein (SNCA Human)

Tags

6xHis Tag and TEV Cleavage Site

Expression

Bacterial

Promoter

T7

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a-C2m2-SNAP

Plasmid

#208773

Purpose

To express C2m2-SNAP in bacteria

Depositor

Urte Neniskyte

Publication

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-SNAP (Mfge8 Mouse)

Tags

SNAP-tag

Expression

Bacterial

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

T7 promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-MED1LCD

Plasmid

#204404

Purpose

E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Publication

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

MED1(947-1581) (MED1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-CoilR-Cys

Plasmid

#181989

Purpose

Used to label proteins with the CoilE genetically encoded tag

Depositor

Kimberly Beatty

Publication

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

CoilR-Cys

Tags

His-Tag

Expression

Bacterial

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-miniE-DBCO

Plasmid

#181985

Purpose

Used to label proteins with the MiniR genetically encoded tag

Depositor

Kimberly Beatty

Publication

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniE-DBCO

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-8

Plasmid

#172772

Purpose

Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-NAD-cpLuc2

Plasmid

#135629

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

Depositor

Kai Johnsson

Publication

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-cpLuc2 sensor

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-GpNLuc-6xHis

Plasmid

#135932

Purpose

Expresses the GpNLuc LumiFluor in bacterial cells.

Depositor

Antonio Amelio

Publication

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p37)

Plasmid

#135939

Purpose

Expresses AUF1p37 in E. coli

Depositor

Yimon Aye

Publication

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Brg1 Del3 pET28a

Plasmid

#122117

Purpose

Expression Brg1 amino acids 836-1446 in bacteria

Depositor

Paul Herring

Publication

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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