We narrowed to 9,981 results for: pcDNA3.1(+)

1. pcDNA3.1-AntiHer2-LC122-TAG

   Plasmid

   #226839

   Purpose

   Contains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

   Inserts

   anti-HER2 light chain
   anti-HER2 heavy chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   amino acid 122 of light chain mutated to TAG

   Promoter

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-AntiHer2-HC121-TAG

   Plasmid

   #226836

   Purpose

   Contains trastuzumab with a TAG codon at position 121 of the heavy chain for ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

   Inserts

   anti-HER2 light chain
   anti-HER2 heavy chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   amino acid 121 of heavy chain mutated to TAG

   Promoter

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-AntiHer2-HC197-TAG

   Plasmid

   #226838

   Purpose

   Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

   Inserts

   anti-HER2 light chain
   anti-HER2 heavy chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   amino acid 197 of heavy chain mutated to TAG

   Promoter

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

   Plasmid

   #187727

   Purpose

   Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L with residues 226-382 removed (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   deletion of residues 226-382

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

   Plasmid

   #187728

   Purpose

   Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L with residues 418-495 removed (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   deletion of residues 418-495

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-myc-GFP-TNRC6A-SD-WT

   Plasmid

   #215897

   Purpose

   Expression of TNRC6A-silencing domain (SD)

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-Myc-BirA*-CAMK2D

   Plasmid

   #221686

   Purpose

   Mammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioID

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   CAMK2D (CAMK2D Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+) EGFP-RalA(G23C)

   Plasmid

   #224275

   Purpose

   cell transfection and expreswsion of RalA (G23C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   RalA (RALA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G23C

   Promoter

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-Myc-BirA*-RNF8

   Plasmid

   #221683

   Purpose

   Mammalian expression of Myc-tagged BirA*-RNF8 fusion protein for BioID

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   RNF8 (RNF8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-39S Light chain

    Plasmid

    #216297

    Purpose

    Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    39S Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-MF3958 Light chain

    Plasmid

    #216304

    Purpose

    Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    MF3958 Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-H2-18 Heavy chain-SpyTag003

    Plasmid

    #216307

    Purpose

    Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-GST-EGFP-GPIDAF

    Plasmid

    #213722

    Purpose

    For overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an SV40 promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    Tags

    V5-tag-GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-GST-EGFP-GPICEAM7

    Plasmid

    #213723

    Purpose

    For overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    Tags

    V5-tag-GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

    Plasmid

    #214614

    Purpose

    adapter for RTK split TEV recruitment assays

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    3xSH2 domains of GRB2-CTEV (GRB2 Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-TST-EGFP-GPIDAF

    Plasmid

    #213712

    Purpose

    For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    Tags

    Twin-strep-tag and V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-TST-EGFP-GPIBY55

    Plasmid

    #213711

    Purpose

    For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    Tags

    Twin-strep-tag and V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-VcnTS-E1015A-E1021A

    Plasmid

    #213417

    Purpose

    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-VcnCS-E1015A-E1021A

    Plasmid

    #213414

    Purpose

    Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1015A-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid

    #208647

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge