We narrowed to 9,934 results for: plasmid dna

1. pBV-Luc

   Plasmid

   #16539

   Purpose

   luciferase reporter plasmid with very low basal activity

   Depositor

   Bert Vogelstein

   Article

   He et al Cell. 1999 Oct 29. 99(3):335-45.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIS-GALkFLP(URA)

   Plasmid

   #49460

   Purpose

   overespression of Flp step-arrest mutant in yeast, URA selection

   Depositor

   Marc Gartenberg

   Article

   Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   GAL10

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBIS-GALkFLP(Trp1)

   Plasmid

   #49459

   Purpose

   overespression of Flp step-arrest mutant in yeast, TRP1 selection

   Depositor

   Marc Gartenberg

   Article

   Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   GAL10

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMKO.1 GFP

   Plasmid

   #10676

   Purpose

   Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

   Depositor

   William Hahn

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. TB:7xSMAD_RE:MiniP

   Plasmid

   #133879

   Purpose

   7 copies the SMAD DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   7 copies the SMAD DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. hisSUMO-ZFP57

   Plasmid

   #102716

   Purpose

   Express DNA binding domain of human Zinc finger protein 57 in E. coli

   Depositor

   Gary Stormo

   Article

   Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

   Insert

   Zinc finger protein 57 (ZNF57 Human)
   

   Use

   Tags

   hisSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TB:2xP53_RE:MiniP

   Plasmid

   #133881

   Purpose

   2 copies of the p53 DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   2 copies of the p53 DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pspCas9-Klenow-ST2-com-vector

   Plasmid

   #176235

   Purpose

   Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pspCas9-POLI-ST2-com vector

   Plasmid

   #176234

   Purpose

   Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET16B.Pfu

    Plasmid

    #12509

    Purpose

    Bacterial expression of Pfu DNA polymerase

    Depositor

    Sung-Hou Kim

    Article

    Goldman et al Acta Crystallogr D Biol Crystallogr. 199

    Insert

    Pfu DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y218H, Q462R, F533L, K559I and V683A (mutations w…

    Promoter

    Available since

    Sept. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAP1638

    Plasmid

    #137089

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    4*(GFP11)
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAP1836

    Plasmid

    #137087

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAP1837

    Plasmid

    #137086

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAP3041

    Plasmid

    #137083

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFP

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    GFP11::3xFlag::tagRFP
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAP1680

    Plasmid

    #137088

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFP

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    tagRFP
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJSM101

    Plasmid

    #48791

    Purpose

    NHEJ reporter plasmid for 5' and 3' cohesive ends

    Depositor

    Michael Glickman

    Article

    Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

    Insert

    split lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJSM103

    Plasmid

    #48872

    Purpose

    NHEJ reporter plasmid for blunt ends

    Depositor

    Michael Glickman

    Article

    Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

    Insert

    split lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CAG-GFPd2

    Plasmid

    #115665

    Purpose

    Piggybac transposon plasmid for destabilized eGFP (GFPd2)

    Depositor

    Jordan Green

    Article

    Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

    Insert

    GFPd2
    

    Use

    Synthetic Biology

    Tags

    Destabilization

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. psPAX2-D64V

    Plasmid

    #63586

    Purpose

    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

    Depositor

    Andrew Scharenberg, David Rawlings

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Packaging plasmid for integrase deficient lentiviral vectors
    

    Use

    Integrase deficient lentiviral packaging vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pexA2_ring

    Plasmid

    #89672

    Purpose

    Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the ring shown in figure 3C of associated article.

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    ring
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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