We narrowed to 3,466 results for: ttl

1. pOCC61

   Plasmid

   #118877

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   mGFP, HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC72

   Plasmid

   #118866

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease, mGFP, cleavable…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOCC28

   Plasmid

   #118873

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE331

   Plasmid

   #153240

   Purpose

   M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

   Plasmid

   #191627

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQCas12j2

   Plasmid

   #189780

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Insert

   Cas12j2
   

   Use

   CRISPR; Gateway compatible cas12j2 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR221-PGK.Pro

   Plasmid

   #79489

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   PGK Promoter (PGK1 Human, Synthetic)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ad-CBR (GFP/cAPC)

   Plasmid

   #16576

   Depositor

   Bert Vogelstein

   Article

   Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

   Insert

   cAPC (APC Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residues 958-2076

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pOCC267

   Plasmid

   #118887

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6-MBP, cleavable with 3C protease; HALO tag

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ230-RV

    Plasmid

    #188544

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RV
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC167

    Plasmid

    #118909

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. gCOTS-pyl

    Plasmid

    #92048

    Purpose

    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Cyanobacteria s. elongatus genome recombination i…

    Tags

    Expression

    Mutation

    Promoter

    LeuP (native S. elongatus promoter) and PrcbL

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOEM-GED-EGFP

    Plasmid

    #102782

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, eGFP

    Depositor

    Elly Tanaka

    Article

    Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

    Insert

    EGFP
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC93

    Plasmid

    #118911

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Use

    Tags

    eGFP-HIS6

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBEG R2-iCreT2ALuc-L3 #BGV208

    Plasmid

    #48992

    Purpose

    Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Inserts

    cre recombinase
    Luciferase
    

    Use

    Luciferase; Gateway cloning vector

    Tags

    IRES (weak)

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pOCC121

    Plasmid

    #118893

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and MBP, cleavab…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGE663

    Plasmid

    #153261

    Purpose

    M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge