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pPtet-2-VP16

Plasmid

#213842

Purpose

Used for expressing VP16 fused to ZF-2.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1-VP16

Plasmid

#213843

Purpose

Used for expressing VP16 fused to ZF-1.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-STReTCh-RGD

Plasmid

#216532

Purpose

Expresses HaloTag-STReTCh-RGD fusion protein in bacteria

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

HaloTag-STReTCh-RGD

Tags

6xHis and HaloTag

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pRegev_D3

Plasmid

#204182

Purpose

Single reporter with long guanine depleted (G-poor) region from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D1.61C>G

Plasmid

#204179

Purpose

pRegev_D1 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D1 with 61C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

61C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S5

Plasmid

#204174

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S5 in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D2.30C>G

Plasmid

#204181

Purpose

pRegev_D2 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D2 with 30C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

30C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S1

Plasmid

#204150

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S1 exon in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S4

Plasmid

#204170

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon S4 in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_S2

Plasmid

#204162

Purpose

Single reporter with secondary structure from splicing library.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

S2 exon in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D4.34C>G

Plasmid

#204185

Purpose

pRegev_D4 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D4 with 34C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

34C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_D3.48C>G

Plasmid

#204183

Purpose

pRegev_D3 with single C>G mutation.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Exon D3 with 48C>G mutation in beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Mutation

48C>G mutation

Promoter

Truncated CAG

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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SL-TREAT

Plasmid

#196918

Purpose

The TREAT reporter containing cap-proximal GC-rich stemloop

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(G41)

Plasmid

#197939

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(G41S)

Plasmid

#197940

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(V97T)

Plasmid

#197941

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pProbe_CUSPER

Plasmid

#185953

Purpose

broad host plasmid that can be used to construct reproductive success bioreporter

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al ISME J. 2023 Sep;17(9):1445-1454. doi: 1

Insert

Unspecified

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSMNot_sra-6::trp-4

Plasmid

#182261

Purpose

trp-4 cDNA cloned into pSMNot_sra-6 at Kpn&Notl

Depositor

Sreekanth Chalasani

Article

Ibsen et al Nat Commun. 2015 Sep 15;6:8264. doi: 10.

Inserts

trp-4 (trp-4 Nematode)
sra-6 (sra-6 Nematode)

Expression

Bacterial

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSecEffector wo TEVp

Plasmid

#172892

Purpose

atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

Bxb1
TP901

Expression

Bacterial

Promoter

nTETO and pBAD /AraC

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSMNot_odr-3::trp-4

Plasmid

#182262

Purpose

trp-4 cDNA cloned into pSMNot_odr-3 at KpnI & NotI

Depositor

Sreekanth Chalasani

Article

Ibsen et al Nat Commun. 2015 Sep 15;6:8264. doi: 10.

Inserts

trp-4 (trp-4 Nematode)
odr-3 (odr-3 Nematode)

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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