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We narrowed to 63,812 results for: cloning

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Showing: 2761 - 2780 of 63812 results

  3. p2R3z-BASI-ccdB-BSAI

    Plasmid
    #118389
    Purpose
    Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Publication
    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4
    Insert
    ccdB
    Use
    Unspecified
    Mutation
    See Depositor Comments Section
    Available since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118389-plasmid-map-sequence-id-470418

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  4. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74492-plasmid-map-sequence-id-140493

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  5. HUWE1

    Plasmid
    #25211
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    huwe1.4005.4374 (HUWE1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    HECT domain
    Available since
    Feb. 18, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  6. pLIX403-ccdB-Blast

    Plasmid
    #158560
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158560-plasmid-map-sequence-id-309876

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  9. pRL1342

    Plasmid
    #70691
    Purpose
    RSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.
    Depositor
    Peter Wolk
    Publication
    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.
    Type
    Empty backbone
    Use
    Bacterial shuttle vector
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70691-plasmid-map-sequence-id-126468

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  12. pVITRO1-102.1F10-IgG2/λ

    Plasmid
    #50367
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Expression
    Mammalian
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50367-plasmid-map-sequence-id-368192

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  15. pVITRO1-102.1F10-IgM/λ

    Plasmid
    #61880
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61880-plasmid-map-sequence-id-521607

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  17. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Keith Joung
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140082-plasmid-map-sequence-id-292704

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  19. pVITRO1-102.1F10-IgG3/λ

    Plasmid
    #50368
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Expression
    Mammalian
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50368-plasmid-map-sequence-id-368212

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Showing: 2761 - 2780 of 63812 results