Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 63,044 results for: cloning

Clear keywords
Showing: 2761 - 2780 of 63044 results

  1. pVITRO1-dV-IgE/κ

    Plasmid
    #61878
    Purpose
    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61878-plasmid-map-sequence-id-101191

    Enlarge

  5. PKM2 (3GQY)

    Plasmid
    #25360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PKM2_1 (PKM Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  6. DNMT3A

    Plasmid
    #25328
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DNMT3A_11 (DNMT3A Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25328-plasmid-map-sequence-id-356911

    Enlarge

  7. mMaroon-N1

    Plasmid
    #54554
    Purpose
    Localization: N1 Cloning Vector, Excitation: 610, Emission: 652
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mMaroon
    Expression
    Mammalian
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    54554-plasmid-map-sequence-id-475388

    Enlarge

  8. pHTNW

    Plasmid
    #136403
    Purpose
    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Publication
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136403-plasmid-map-sequence-id-272878

    Enlarge

  10. mPA-GFP-N1

    Plasmid
    #54712
    Purpose
    Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mPA-GFP
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  12. pRL1383a

    Plasmid
    #70692
    Purpose
    RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.
    Depositor
    Peter Wolk
    Publication
    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.
    Type
    Empty backbone
    Use
    Bacterial shuttle vector
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70692-plasmid-map-sequence-id-126476

    Enlarge

  13. pHH21-BNA-Luc

    Plasmid
    #187924
    Purpose
    Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells
    Depositor
    Arindam Mondal
    Publication
    Kedia et al Front Microbiol. 2022 May 25;13:868367.
    Insert
    Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
    Expression
    Mammalian
    Promoter
    RNA polymease I Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187924-plasmid-map-sequence-id-373471

    Enlarge

  14. CBX7

    Plasmid
    #25241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX7_04 (CBX7 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    4 Articles

  16. pVITRO1-102.1F10-IgA1/λ

    Plasmid
    #50370
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Expression
    Mammalian
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50370-plasmid-map-sequence-id-75287

    Enlarge

  17. RALGDS

    Plasmid
    #25331
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RALGDS:N793-F914 (RALGDS Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids N793-F914
    Available since
    Sept. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  18. RAB1A

    Plasmid
    #25521
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB1A (RAB1A Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. SHMT2

    Plasmid
    #25479
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SHMT2 (SHMT2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    A269T
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    25479-plasmid-map-sequence-id-168679

    Enlarge

  20. CBX4

    Plasmid
    #25237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX4_02 (CBX4 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles
Showing: 2761 - 2780 of 63044 results