We narrowed to 4,290 results for: AES

Showing: 391 - 420 of 4290 results

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pTXTL-T7max-RLuc

Plasmid

#188312

Purpose

Expression of Renilla luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Renilla luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pNCS YFP

Plasmid

#91759

Purpose

Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

Depositor

Erik Rodriguez, Roger Tsien

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Venus YFP (A206K)

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDN-Cherry-sgRNA

Plasmid

#221387

Purpose

episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

none, cloning vector for introduction of targeting sequences

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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PU6::unc-119_sgRNA

Plasmid

#46169

Purpose

unc-119 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

unc-119 targeting sgRNA (unc-119 Synthetic)

Use

CRISPR

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163076

Purpose

Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDN-Sth1Cas9

Plasmid

#221386

Purpose

Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

Sth1 Cas9

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRF0G-Tyr

Plasmid

#113644

Purpose

Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

MjYRS and tRNA gene

Use

Synthetic Biology

Available Since

July 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163075

Purpose

Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Plasmid

#163077

Purpose

Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Peredox

Use

Gateway entry vector

Tags

mCherry

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-ER-ZapCY1

Plasmid

#36321

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ER-ZapCY1 high affinity Zn(II) sensor

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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OSCA3.1

Plasmid

#136596

Purpose

Expresses the mechanosensitive channel OSCA3.1 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA3.1 (ERD4 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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mStrawberry-N1

Plasmid

#54644

Purpose

Localization: N1 Cloning Vector, Excitation: 574, Emission: 596

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Type

Empty backbone

Tags

mStrawberry

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-EGFP

Plasmid

#233208

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pWS3799-gRNA-Csy4-template

Plasmid

#182827

Purpose

SpCas9 gRNA-Csy4 template

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

SpCas9 gRNA scaffold + Csy4 sequence

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5397-CRISPRai-NatR

Plasmid

#182836

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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LiRT

Plasmid

#214075

Purpose

Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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LiRT_RTctrl

Plasmid

#214078

Purpose

Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRYG

Plasmid

#113642

Purpose

Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

mRFP1-linker-sfGFP

Use

Synthetic Biology

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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OSCA1.2

Plasmid

#136593

Purpose

Expresses the mechanosensitive channel OSCA1.2 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA1.2 (AT4G22120 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hRabin8 WT

Plasmid

#26726

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pWS5321-CRISPRai-Markerless

Plasmid

#182828

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - markerless

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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HK_13_Bla_Lenti_Kir2.1

Plasmid

#136632

Purpose

encodes Kir2.1 potassium channel for lentiviral transduction

Depositor

Adam Cohen

Article

McNamara et al Nat Phys. 2020 Mar;16(3):357-364. doi: 1

Insert

Kir2.1

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pWS5399-CRISPRai-ZeoR

Plasmid

#182838

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-EGFP-T2A-Flpo

Plasmid

#233212

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP-T2A-Flpo for selection

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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LZF40: hSyn-Cre

Plasmid

#104124

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

Cre

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-FRB-cTEVP

Plasmid

#179676

Purpose

ER-associated FRB split-cTEVP (Rapalog inducible)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

FRB (MTOR Synthetic, Human)

Tags

cTEVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163073

Purpose

Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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CA-0030 CMVTO-Renin-ECL

Plasmid

#231189

Purpose

Membrane-bound human renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin-ECL (REN Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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DL145_plenti_TRE_iPAK4

Plasmid

#187445

Purpose

Tet-On inducible expression of iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box-Pak4

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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OSCA1.1

Plasmid

#136592

Purpose

Expresses the mechanosensitive channel OSCA1.1 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA1.1 (AT4G04340 Mustard Weed)

Tags

pIRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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