We narrowed to 346 results for: 1266

1. TL1 alphaL_DdCBE 1 (left, DddA11-C)

   Plasmid

   #221996

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TL1 alphaL_DdCBE 4 (right, DddA11-N)

   Plasmid

   #221999

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-4 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   DddA: S1330I, A1341V, N1342S, E1370K, T1380I

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TL1 alphaDdCBE 1-2 (right, DddA11-N)

   Plasmid

   #222000

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-1/2 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | TALE: S1330I, A1341V,…

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TL1 alphaDdCBE 2 (left, DddA11-C)

   Plasmid

   #222001

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-2 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TL1 alphaDdCBE 3_CN (left, DddA11-C)

   Plasmid

   #222002

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-MBP-TEVpCS-ADGRF1 CTF-FLAG

   Plasmid

   #217694

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRF1 (ADGRF1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-566

   Promoter

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. enIscB

   Plasmid

   #205410

   Purpose

   Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E85R+H369R+S387R+S457R

   Promoter

   CAG, hU6, CMV

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJL NarL YdfI Flag

   Plasmid

   #225650

   Purpose

   Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NarL-YdfI (narL Synthetic)
   

   Use

   Cell free expression

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   NarL with native DNA-binding domain replaced with…

   Promoter

   T7

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSC50

   Plasmid

   #104824

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.08g081600, Glyma.05g126600
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3HM1Luc4

    Plasmid

    #236277

    Purpose

    Mammalian expression of human muscarinic M1 receptor; N-terminal HA tag, C-terminal Rluc8

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

    Insert

    muscarinic M1 receptor (CHRM1 Human)
    

    Use

    Tags

    3xHA and Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-Oct3/4-IRES-Neo

    Plasmid

    #13461

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P31S

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)

    Plasmid

    #221998

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-C1-mApg5

    Plasmid

    #22958

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI-neo-mApg5

    Plasmid

    #22956

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

15. TL1 alphaDdCBE 3_CN (right, DddA11-N)

    Plasmid

    #222003

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TL1 alphaDdCBE 3_NC (left, DddA11-N)

    Plasmid

    #222004

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC left)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3xFLAG-CMV10-mApg16L

    Plasmid

    #24302

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

    Insert

    autophagy related genes 16L (Atg16l1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V583M

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. enIscB-T5E

    Plasmid

    #205411

    Purpose

    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E85R+H369R+S387R+S457R

    Promoter

    CAG, hU6, CMV

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. miCBE

    Plasmid

    #205413

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D61A+E85R+H369R+S387R+S457R

    Promoter

    hU6, CAG, CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hSUMO1 GFP

    Plasmid

    #118360

    Purpose

    This plasmid expresses the full length human SUMO1.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hSUMO1 (SUMO1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge