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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,017 results for: HTA

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Showing: 261 - 280 of 1017 results

  2. pcDNA3-PM-ExRai-CKAR2

    Plasmid
    #236098
    Purpose
    Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    Lyn-ExRai-CKAR2
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236098-plasmid-map-sequence-id-473738

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  4. pcDNA3-Lyn-dPlcR

    Plasmid
    #181832
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-PlcR
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181832-plasmid-map-sequence-id-364041

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  8. pcDNA3-Cyto-ExRai-CKAR2

    Plasmid
    #236097
    Purpose
    Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    ExRai-CKAR2-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236097-plasmid-map-sequence-id-473746

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  13. pcDNA3-nuc-Nb80

    Plasmid
    #205519
    Purpose
    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb80
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205519-plasmid-map-sequence-id-409422

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  15. pcDNA3-DAKAP-μDpTT

    Plasmid
    #182324
    Purpose
    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DAKAP-μDronpa-TagRFP-T
    Tags
    N-terminal 30 amino acids from DAKAP1
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182324-plasmid-map-sequence-id-362788

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  18. pcDNA3-Lyn-μDpTT

    Plasmid
    #182321
    Purpose
    Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Lyn-μDronpa-TagRFP-T
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182321-plasmid-map-sequence-id-362887

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  19. pcDNA3-CYP450-μDpTT

    Plasmid
    #182325
    Purpose
    Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DAKAP-μDronpa-TagRFP-T
    Tags
    N-terminal 27 amino acids from CYP450
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182325-plasmid-map-sequence-id-362816

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  20. pcDNA3-Lifeact-μDpTT

    Plasmid
    #182322
    Purpose
    Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Lifeact-μDronpa-TagRFP-T
    Tags
    N-terminal 17 amino acids from budding yeast Abp1…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182322-plasmid-map-sequence-id-362772

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Showing: 261 - 280 of 1017 results