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Plasmid#22380DepositorInsertSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
UseTagsHis and mycExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-mCherry
Plasmid#126667PurposeInducible expression of Draper-mCherry in S2 cellsDepositorInsertDraper mCherry
UseTagsExpressionInsectMutationC813Y- see depositor comments belowPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
aCE121
Plasmid#204492PurposeCadherin circuitDepositorInsertsEBFP2
Cdh6-mKate2
Cdh1-EYFP
UseSynthetic BiologyTags2AExpressionBacterial and MammalianMutationPromoterhEF1aAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(RK)
Plasmid#27136DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationArginines in the SH2 domains mutated to lysines (…PromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3
Plasmid#21800DepositorInsertPSMD14 (Psmd14 Mouse)
UseTagsGST and TEVExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S454A
Plasmid#19844DepositorInsertMKL1 S454A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 454 to AlaninePromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-5p
Plasmid#103366PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-5p target (MIR221 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A
Plasmid#74606Purposeexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dyneinDepositorInsertfull length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationY101A, Y103A, W120APromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 Y101A, Y103A
Plasmid#74605Purposeexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dyneinDepositorInsertfull length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationY101A, Y103APromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-186-5p
Plasmid#103289PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-186-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-186-5p target (MIR186 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4GALT1-Dox
Plasmid#124633PurposeDoxycycline-inducible B4GALT1 expression in mammalian cellsDepositorInsertsBeta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJTX-GL3
Plasmid#11981PurposeReporter gene with mouse c-jun promoter and point mutation of AP1 binding siteDepositorInsertc-jun promoter (Jun Mouse)
UseLuciferaseTagsLucExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE MKL1-N100 S449A/T450A/S454A
Plasmid#19849DepositorInsertMKL1-N100 S449A/T450A/S454A (MRTFA Human)
UseRetroviralTags3xFLAGExpressionMutationdeleted amino acids 1-100 and changed Serine 449,…PromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCHOP10(dLZ).pCDNA1
Plasmid#21900DepositorInsertCHOP10 (Ddit3 Mouse)
UseTagsExpressionMammalianMutationThe coding region of the murine CHOP-10 cDNA was …PromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
TLS 4: hTLS-CHOP.pBSKS(+)
Plasmid#21830DepositorInsertTLS-CHOP (FUS Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-3p
Plasmid#103365PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-3p target (MIR221 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-mIFP-NLS
Plasmid#173893Purposefor mIFP expression in nucleusDepositorInsertmIFP
UseLentiviralTagsExpressionMutationPromoterSSFVAvailable sinceNov. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-191-5p
Plasmid#103300PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-191-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-191-5p target (MIR191 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mP58.dJ1-pCDNA3
Plasmid#21884DepositorInsertP58 (Dnajc3 Mouse)
UseTagsExpressionMammalianMutationDnaJ domain deleted from C-termPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only