Addgene

We narrowed to 1,124 results for: ncl

Showing: 261 - 280 of 1124 results

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pCR8_TaNAM-A1(1-217)

Plasmid

#124576

Purpose

NAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TaNAM-A1 (1-217)

Use

Topo gateway cloning vector

Tags

Expression

Mutation

Promoter

None

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8_TtNAM-B1(1-215)

Plasmid

#124577

Purpose

NAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TtNAM-B1 (1-215)

Use

Topo gateway cloning vector

Tags

Expression

Mutation

Promoter

None

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2001

Plasmid

#105871

Purpose

High copy HRPT backbone plasmid for insertion of MiniP promoters

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-HA-Ubiquitin-K48R

Plasmid

#17604

Purpose

Mammalian expression of HA tagged ubiquitin K48R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K48R (expected to disrupt G76-K48 ubiquitin chain…

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pSpCas9_U6_sgRNA_Mettl3C

Plasmid

#165420

Purpose

sgRNA construct for targeting Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-SIRT1

Plasmid

#1791

Purpose

Mammalian expression of flag-tagged SIRT1

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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Flag-SIRT1 H363Y

Plasmid

#1792

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H363Y, deacetylase domain mutation

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-hAtg12

Plasmid

#87869

Purpose

Express a EGFP-human Atg12/Apg12 in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG12 (ATG12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-FOXO3a WT

Plasmid

#8360

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

FOXO3a (FOXO3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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FLAG-FOXO3a TM

Plasmid

#8361

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

FOXO3a (FOXO3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T32A, S253A, S315A

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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p6345 MSCV-CMV-Flag-HA-Brd4 1-722

Plasmid

#31352

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

*see comments

Promoter

Available since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-K179M

Plasmid

#73409

Purpose

Expresses kinase-inactive mutant of Akt with HA tag

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

changed K179M to make Kinase inactive mutant

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGABARAP

Plasmid

#87871

Purpose

Express a EGFP-human GABARAP in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

GABARAP (GABARAP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(D3A: D175A D28A D9A)

Plasmid

#90090

Purpose

Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

Depositor

Clay Clark

Article

Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa1-149

Plasmid

#73410

Purpose

Expresses HA tagged aa1-149 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-149 only of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-Akt1-aa120-433

Plasmid

#73411

Purpose

Expresses HA tagged aa120-433 AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 120-433 of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-4mtD3cpv

Plasmid

#181874

Purpose

Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

4mtD3cpv

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

the 4mtD3cpv CDS differs from the recorded GeneBa…

Promoter

CaMKIIa

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_Mm_CEP55(WT)

Plasmid

#136333

Purpose

Mouse CEP55 cDNA in Gateway pENTR

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Commun. 2018 Aug 1;9(1):3012. doi: 1

Insert

CEP55 (Cep55 Mouse)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa1-408

Plasmid

#73412

Purpose

Expresses HA tagged aa1-408 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-408 of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only