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We narrowed to 6,508 results for: nls

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Showing: 261 - 280 of 6508 results

  2. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47455
    Purpose
    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    Use
    AAV
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47455-plasmid-map-sequence-id-69627

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  3. pDAC569

    Plasmid
    #195241
    Purpose
    Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    Expression
    Mammalian
    Available Since
    Feb. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195241-plasmid-map-sequence-id-384464

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  6. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh, H1, and U6
    Available Since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    64217-plasmid-map-sequence-id-123057

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  12. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Available Since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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  15. pAAV-mDlx-NLS-mRuby2 (AAV1)

    Viral Prep
    #99130-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.
    Promoter
    mDLX5/6
    Tags
    mRuby2
    Available Since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. pCSN072

    Plasmid
    #166730
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166730-plasmid-map-sequence-id-332216

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  20. pAAV-mDlx-NLS-mRuby2 (AAV9)

    Viral Prep
    #99130-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.
    Promoter
    mDLX5/6
    Tags
    mRuby2
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 261 - 280 of 6508 results