We narrowed to 13,054 results for: sequence

1. pcDNA3-RsSHC014-RBD-8h

   Plasmid

   #161826

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR P2R-P3-EGFP

   Plasmid

   #48349

   Purpose

   Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Aminoacid 40 is valine in our sequence, while it …

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Rs7327-RBD-8h

   Plasmid

   #161823

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Rs4084-RBD-8h

   Plasmid

   #161825

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-Rs4231-RBD-8h

   Plasmid

   #161824

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T320-D518)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX459_HR_Prnp_3

   Plasmid

   #78623

   Purpose

   pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

   Insert

   HR_Prnp_3 (Prnp Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKrox24(MapErk)DsRed

   Plasmid

   #200114

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   MapErk sequence in minimal promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   5 copies of designed MapErk sequence, no other pr…

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-H2B

   Plasmid

   #108883

   Purpose

   Multisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   H2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)
   

   Use

   Multisite gateway

   Tags

   Expression

   Mutation

   human amino acid sequence but different codon usa…

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet-solo-NHis6-NocO-H286A

   Plasmid

   #240409

   Purpose

   expresses N-His tagged NocO H286A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

   Depositor

   Emily Balskus

   Article

   Wang et al Biochemistry. 2025 May 20;64(10):2173-21

   Insert

   nocO
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M1 of native sequence not included in insert, His…

   Promoter

   T7

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETDuet-solo-NHis6-NocO-H132A

    Plasmid

    #240404

    Purpose

    expresses N-His tagged NocO H132A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, His…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDuet-solo-NHis6-NocO-E129A

    Plasmid

    #240403

    Purpose

    expresses N-His tagged NocO E129A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETDuet-solo-NHis6-NocO-Q128A

    Plasmid

    #240402

    Purpose

    expresses N-His tagged NocO Q128A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETDuet-solo-NHis6-NocO-E252A

    Plasmid

    #240405

    Purpose

    expresses N-His tagged NocO E252A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETDuet-solo-NHis6-NocO-E283A

    Plasmid

    #240408

    Purpose

    expresses N-His tagged NocO E283A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETDuet-solo-NHis6-NocO-H279A

    Plasmid

    #240406

    Purpose

    expresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, His…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETDuet-solo-NHis6-NocO-E94A

    Plasmid

    #240401

    Purpose

    expresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAGGS-Flex/3'USS(103)-GFP

    Plasmid

    #197890

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAGGS-Flex/3'USS(553)-GFP

    Plasmid

    #197888

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAGGS-Flex/3'USS(285)-GFP

    Plasmid

    #197889

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2 mir-1-1 reporter mir129-2-2

    Plasmid

    #46728

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir129-2-2 (MIR129-2 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge