We narrowed to 11,100 results for: CHL

1. pTriEx-mCherry-Zdk1-Rac1 Q61L

   Plasmid

   #81059

   Purpose

   mammalian expression of Zdk1 tagged Rac1 Q61L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   Rac1 Q61L (RHOA Human)
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pTriEx-mVenus-Zdk1-RhoA Q63L

   Plasmid

   #81046

   Purpose

   mammalian expression of Zdk1 tagged RhoA Q63L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   RhoA Q63L (RHOA Human)
   

   Tags

   mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriex-mVenus-PI-RhoA(Q63L)

   Plasmid

   #86978

   Purpose

   PI(DM): Photo-activatable/light sensitive

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   RhoA (RHOA Human)
   

   Tags

   6xHis and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q63L

   Promoter

   CMV

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-mVenus-Zdk1-Rac1 Q61L

   Plasmid

   #81137

   Purpose

   mammalian expression of Zdk1 tagged Rac1 Q61L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   Rac1 (RHOA Human)
   

   Tags

   mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q61L

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMDC32B-AtTAS1c-D2-B/c

   Plasmid

   #137884

   Purpose

   Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]

   Depositor

   Alberto Carbonell

   Article

   Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

   Insert

   AtTAS1c-D2-B/c
   

   Expression

   Plant

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   2x35S

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV CAG ChR2 E123T T159C 2A tDimer

   Plasmid

   #85399

   Purpose

   High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

   Depositor

   Thomas Oertner

   Article

   Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E123T , T159C

   Promoter

   CAG and ribosomal skip sequence 2A

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 myc card9 mutant

   Plasmid

   #16254

   Depositor

   William Kaelin

   Article

   Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

   Insert

   card9 mutant (CARD9 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S528A, T531A, T533A, S536A

   Available Since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pET24a-VHH-tev-std

   Plasmid

   #109423

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO hChR2(E123A)-mCherry

    Plasmid

    #35508

    Purpose

    AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-CERT-PH

    Plasmid

    #49572

    Purpose

    expresses the plekstrin homology domain of CERT/OSBP in mammalian cells

    Depositor

    Marci Scidmore

    Article

    Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

    Insert

    GPBP (CERT1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expresses AA 1-122

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

12. hSyn-somAion-citrine

    Plasmid

    #192570

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    somAion
    

    Use

    AAV

    Tags

    Citrine - soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pROF141

    Plasmid

    #155329

    Purpose

    Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo

    Plasmid

    #179439

    Purpose

    Expresses rTetR-KRAB(human) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-KRAB
    

    Expression

    Mammalian

    Promoter

    pGK

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-HA-TEV

    Plasmid

    #105769

    Purpose

    Expression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    HA-TEV

    Expression

    Mammalian

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. eGFP-KCC2

    Plasmid

    #104075

    Purpose

    expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

    Depositor

    Igor Medyna

    Article

    Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    CMV

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKK-TEV-HA

    Plasmid

    #105785

    Purpose

    Expression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-HA

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. L-CRISPR-CTN (MLL-ctrl)

    Plasmid

    #69215

    Purpose

    Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV promoter
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX307 ARHGAP30

    Plasmid

    #117738

    Purpose

    Open reading frame vector encoding ARHGAP30

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    ARHGAP30 (ARHGAP30 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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