We narrowed to 9,547 results for: SUB

1. pP2A-mCherry-N1

   Plasmid

   #84329

   Purpose

   Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   P2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-PD-1 G224A-mGFP

   Plasmid

   #180819

   Purpose

   Expression of human PD-1 G224A fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   G224A

   Promoter

   SFFV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-NLS-miRFP670nano-miRFP670

   Plasmid

   #216131

   Purpose

   Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS26_QUAS_CsChrimson

   Plasmid

   #175548

   Purpose

   Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

   Depositor

   Leslie Vosshall

   Article

   Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

   Insert

   CsChrimson
   

   Use

   Tags

   tdTomato

   Expression

   Insect

   Mutation

   Promoter

   QUAS

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 MEKK1 WT

   Plasmid

   #12181

   Depositor

   Gary Johnson

   Article

   Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

   Insert

   MEKK1 (Map3k1 Mouse)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   V1467L

   Promoter

   Available since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Flex-rev-oChIEF-tdTomato

   Plasmid

   #30541

   Purpose

   Cre-dependent viral expression of oChIEF-tdTomato for optogenetic excitation

   Depositor

   Rory McQuiston

   Article

   Bell et al Neuropharmacology. 2011 Aug 25.

   Insert

   oChIEF-tdtomato
   

   Use

   AAV and Cre/Lox

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETDuet RagA-RagC

   Plasmid

   #99641

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBI-CMV1-mir144-CMV2-mir1

   Plasmid

   #164634

   Purpose

   Express miR-144 and miR-1-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   mir144 (MIR144 Human)
   mir-1-1 (MIR1-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-FKBP-PI4KAC1001(D1957A)

    Plasmid

    #139312

    Purpose

    Recruitable PI4KA kinase-dead catalytic fragment

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D1957A, G1986D

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Thy1 promoter construct

    Plasmid

    #20736

    Depositor

    Joshua Sanes

    Article

    Feng et al Neuron. 2000 Oct . 28(1):41-51.

    Insert

    Thy1 promoter (Thy1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-ATXN1-52Q

    Plasmid

    #32492

    Depositor

    Huda Zoghbi

    Article

    Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 52 Glutamines and no…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pFastBac1 Flag BRG1

    Plasmid

    #1957

    Depositor

    Robert Kingston

    Article

    Phelan et al Mol Cell 1999 Feb;3(2):247-53.

    Insert

    BRG1 (SMARCA4 Human)
    

    Use

    Tags

    flag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pILGFPI7

    Plasmid

    #83555

    Purpose

    Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CUP1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS613_pTetR-P2A-BFPnls/sgTelo

    Plasmid

    #108649

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Telomere-targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE60 R130A wgMDH

    Plasmid

    #204266

    Purpose

    Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAM233

    Plasmid

    #226655

    Purpose

    pcDNA3.1-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZac2.1 gfaABC1D-LifeAct-GFP

    Plasmid

    #190199

    Purpose

    Expresses the small actin binding peptide, LifeAct, with a GFP reporter

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    LifeAct-eGFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCX-SpiCee-NES

    Plasmid

    #140901

    Purpose

    mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NES
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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