We narrowed to 9,532 results for: SUB;

1. mCherry-FKBP-PI4KAC1001(D1957A)

   Plasmid

   #139312

   Purpose

   Recruitable PI4KA kinase-dead catalytic fragment

   Depositor

   Gerry Hammond

   Article

   Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D1957A, G1986D

   Promoter

   Available since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-ATXN1-52Q

   Plasmid

   #32492

   Depositor

   Huda Zoghbi

   Article

   Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 52 Glutamines and no…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Thy1 promoter construct

   Plasmid

   #20736

   Depositor

   Joshua Sanes

   Article

   Feng et al Neuron. 2000 Oct . 28(1):41-51.

   Insert

   Thy1 promoter (Thy1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS613_pTetR-P2A-BFPnls/sgTelo

   Plasmid

   #108649

   Purpose

   Telomere-targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Telomere-targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAM233

   Plasmid

   #226655

   Purpose

   pcDNA3.1-p97-FLAG

   Depositor

   Andreas Martin

   Article

   Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

   Insert

   p97 (VCP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE60 R130A wgMDH

   Plasmid

   #204266

   Purpose

   Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130A

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZac2.1 gfaABC1D-LifeAct-GFP

   Plasmid

   #190199

   Purpose

   Expresses the small actin binding peptide, LifeAct, with a GFP reporter

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   LifeAct-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCX-SpiCee-NES

   Plasmid

   #140901

   Purpose

   mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NES
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SDHC-pHLuorin-sems

   Plasmid

   #107767

   Purpose

   labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

   Depositor

   Karin Busch

   Article

   Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

   Insert

   Subunit C of SDH fused to pHluorin (SDHC Human)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFP91

    Plasmid

    #83548

    Purpose

    Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    PGK1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-FKBP-PI4KAC1001

    Plasmid

    #139311

    Purpose

    Recruitable PI4KA catalytic fragment

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G1986D

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YFP-NOP58-C1

    Plasmid

    #134537

    Purpose

    Expresses YFP-NOP58 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    NOP58 (NOP58 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. KG#901

    Plasmid

    #110936

    Purpose

    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET24a_SMOXdel

    Plasmid

    #186785

    Purpose

    C-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cells

    Depositor

    Sandra Gabelli

    Article

    Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

    Insert

    Spermine oxidase (SMOX Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 503-512

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4046887

    Plasmid

    #145730

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pQE60 R124K wgMDH

    Plasmid

    #204258

    Purpose

    Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124K

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQE60 R124Q wgMDH

    Plasmid

    #204259

    Purpose

    Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124Q

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE60 R124A wgMDH

    Plasmid

    #204257

    Purpose

    Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQE60 R130E wgMDH

    Plasmid

    #204267

    Purpose

    Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130E

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge