We narrowed to 34,066 results for: crispr

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

Plasmid

#64113

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLentiCas9-BFP

Plasmid

#78545

Purpose

Fusion protein Cas9+BFP

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pYPQ133A

Plasmid

#69275

Purpose

Golden Gate entry vector; 3rd gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCascade_SPS1(PmcCAST)

Plasmid

#224926

Purpose

Expression and purification of type I-B2 Cascade in CRISPR-associated transposon of Romsetta

Depositor

Leifu Chang

Publication

Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

Inserts

Cas5, Cas6, Cas7, Cas8, Cas11
CRISPR assay for crRNA expression

Use

CRISPR

Expression

Bacterial

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

Plasmid

#179003

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

Plasmid

#178996

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

Plasmid

#178995

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pY026

Plasmid

#84741

Purpose

Expresses huAsCpf1 and crRNA guide

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

huAsCpf1

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pEJS540-pCSDest2-AcrIIC4Hpa

Plasmid

#113434

Purpose

Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

Depositor

Erik Sontheimer

Publication

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Type II-C anti-CRISPR

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEJS542-pCSDest2-AcrIIC5Smu

Plasmid

#113435

Purpose

Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells

Depositor

Erik Sontheimer

Publication

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Type II-C anti-CRISPR

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-mitf

Plasmid

#69801

Purpose

Used to make mitf knockout porcine cell line

Depositor

Jianguo Zhao

Publication

Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

mitf-sgRNA
SpCas9

Expression

Mammalian

Promoter

Chicken Beta-Actin and U6

Available since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDonor_hU6

Plasmid

#69312

Purpose

sgRNA scaffold and human U6 promoter

Depositor

Andrea Ventura

Publication

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

hU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

human U6 promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

Plasmid

#107055

Purpose

Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

Depositor

Alex Hewitt

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

Plasmid

#103869

Purpose

dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ142

Plasmid

#69294

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSECRETS-A

Plasmid

#196986

Purpose

For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

Depositor

Eric Josephs

Publication

Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pltetO1

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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