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pGL2-basic mt E2F-luciferase

Plasmid

#133708

Purpose

Expression of E2F1-mt-luc.

Depositor

James A. DeCaprio

Article

Zalvide et al Mol Cell Biol 1998 Mar;18(3):1408-15.

Insert

E2F1 (E2F1 Human)

Use

Trxn factor bind site

Expression

Mammalian

Mutation

the three binding site copies are mutated form

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc NES-mCE NLS-Flag

Plasmid

#82468

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-NES-mCE-Flag (Rngtt Mouse, HIV)

Tags

Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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HOXA4 (murine) pET

Plasmid

#8488

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXA4 (Hoxa4 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Thr-2 changed to Ala-2 to form better ribosomal b…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
Cerulean(1-158) in pcDNAI/Amp

Plasmid

#60897

Purpose

This vector attaches Cerulean(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

Cerulean(1-158)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf nhsl1b-mNeongreen

Plasmid

#233883

Purpose

mNeongreen tagged form of zebrafish nhsl1b. For in vitro transcription (SP6) or expression through CMV.

Depositor

Nicolas David

Article

Escot et al Commun Biol. 2025 Feb 28;8(1):328. doi:

Insert

nhsl1b (nhsl1b Zebrafish)

Use

In vitro synthesis of mrna

Tags

mNeongreen

Expression

Mammalian

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-myc-ATL3-WPRE-UbC-Emerald

Plasmid

#225946

Purpose

Lentiviral vector plasmid expressing human atlastin GTPase 3 (ATL3) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis, Evan Reid

Inserts

ATL3 (ATL3 Human)
Emerald

Use

Lentiviral

Tags

myc-tag

Expression

Mammalian

Promoter

SFFV and UbC

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

Plasmid

#225949

Purpose

Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis, Evan Reid

Inserts

CKAP4 (CKAP4 Human)
Emerald

Use

Lentiviral

Expression

Mammalian

Mutation

C100A

Promoter

SFFV and UbC

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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mCit(S1h)3(S3h)3-gs

Plasmid

#228079

Purpose

Mammalian expression of coiled coil segments (S1h)x3-(S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, S1h, S3h

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2-S4)1-gs

Plasmid

#228074

Purpose

Mammalian expression of coiled coil segments (S2-S4)x1, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(S1h-S3h)3-gs

Plasmid

#228076

Purpose

Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, S1h, S3h

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(D1h-D3h)3-gs

Plasmid

#228088

Purpose

Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, D1h, D3h

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(S1-S3)1-SAH

Plasmid

#228073

Purpose

Mammalian expression of coiled coil segments (S1-S3)x1, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCitrine, S1, S3

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(S1-S3)3-gs

Plasmid

#228070

Purpose

Mammalian expression of coiled coil segments (S1-S3)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCitrine, S1, S3

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2-S4)3-gs

Plasmid

#228075

Purpose

Mammalian expression of coiled coil segments (S2-S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2, S4

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)3(S4h)3-gs

Plasmid

#228077

Purpose

Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h, S4h

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8_SH2sub6

Plasmid

#175799

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8_SH2sub6

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET-SOMA-T679A

Plasmid

#118936

Purpose

Used to express the T679A mutant form of the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMT2 Cav2.2 DomI and DomII

Plasmid

#89891

Purpose

Mammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

Cacna1b (CACNA1B )

Expression

Mammalian

Mutation

Stop codon after amino acid 1154 (this is a trunc…

Promoter

adenovirus major late promoter

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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YCpADE1

Plasmid

#22494

Depositor

Beth Montelone

Insert

ADE1 (ADE1 Budding Yeast)

Use

Yeast shuttle

Available Since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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