We narrowed to 9,466 results for: CAG

1. pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85038

   Purpose

   For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   FRT-stop-FRT
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)

   Plasmid

   #114095

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-RR (E174R/S542R/K607R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   E174R, S542R and K607R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

   Plasmid

   #179586

   Purpose

   ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

   Depositor

   Ali Hemmati Brivanlou

   Article

   De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

   Insert

   NLS-NG-T2A-hSHH
   

   Use

   Piggybac

   Tags

   NeonGreen

   Promoter

   TRE

   Available Since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85179

   Purpose

   For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nuclear localization signal (nls) tagRFP
   

   Expression

   Mammalian

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

   Plasmid

   #114092

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (S542R/K548V/N552R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R, K548V and N552R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA)

   Plasmid

   #194728

   Purpose

   AAVS1-tdTomato targeting vector, compartible with Cpf1

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   tdTomato
   

   Use

   CRISPR; Knockin targeting plasmid

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-loxP-3xpA-H2BsfGFP-tdTomato - opt GS PQR Var3 P2A -APGST E

   Plasmid

   #113847

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by P2A (-APGST linker)

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-P2A-tdTomato
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEP116[EF1a-mNeonGreen]-pAAVS1-SA-Neo-TRE3Gs-NGN2-2A-Zeo-CAG-TETON3G

   Plasmid

   #243805

   Purpose

   Integrates dox-inducible hNGN2 into the human AASV1 safe harbor locus for directed differentiation of neural cells from stem cells

   Depositor

   Michael Wells

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   TALEN

   Promoter

   TRE3GS

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

   Plasmid

   #231206

   Purpose

   Single transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
   

   Expression

   Mammalian

   Mutation

   L147I, R148H, I203V, L290V

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. CA-0266 CMVTO-IVT-UTR-mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin (FLP-IN)

    Plasmid

    #231209

    Purpose

    Single transcript mRNA cageIL-2 (mRens) + ER-renin (H2M)

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    UTR-mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin (IL2 Human)
    

    Expression

    Mammalian

    Mutation

    L80F, R81D, L85V, I86V, I92F

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9

    Plasmid

    #196897

    Purpose

    Neuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    2xHA-Akap9(128-425) (Akap9 Rat)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid

    #107942

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R, and N282A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #86233

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

    Plasmid

    #92143

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

    Plasmid

    #53959

    Purpose

    allows Cre-mediated expression of transgene

    Depositor

    Jeffrey Robbins

    Article

    Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Lox-CAT-Lox

    Expression

    Mammalian

    Promoter

    CMV enhancer, chicken actin promoter

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)

    Plasmid

    #114093

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548V and N552R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

    Plasmid

    #122955

    Purpose

    Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Mutation

    deletion of EGF repeats from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)

    Plasmid

    #242587

    Purpose

    A CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nlsKateV5, MbBPF-FLAG, FRTNeoFRT
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

    Plasmid

    #197044

    Purpose

    In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

    Depositor

    Liqun Luo

    Article

    Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

    Inserts

    pCAG-FSF-LSL
    SP-HA-HRPtm
    WPRE-bGHpolyA
    

    Tags

    HA, myc

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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