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pLCHKOv3-mClover3

Plasmid

#209034

Purpose

Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mClover3 and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG8H-GB1-EWS_171-264

Plasmid

#180466

Purpose

Expresses the construct His8-GB1-EWS_171-264 with a TEV cleavage site between GB1 and EWS_171-264

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Tags

GB1 and His8

Expression

Bacterial

Mutation

deleted 1-170, 265-656

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAG8H-GFP-EWS_1-120

Plasmid

#180464

Purpose

Expresses the construct His8-GFP-EWS_1-120 with a TEV cleavage site between GFP and EWS_1-120

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Tags

GFP and His8

Expression

Bacterial

Mutation

deleted 121f-656

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-50-DExogron-mCherry-R11b

Plasmid

#179904

Purpose

Donor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMKRQ_11_DExCon-mNeonGreen-R25

Plasmid

#179896

Purpose

Donor with homologous arms for Rab25 to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB25, member RAS oncogene family (RAB25 Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-23-DExCon-mCherry-R11b

Plasmid

#179898

Purpose

Donor with homologous arms for Rab11b to knock in DExCon-mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-67-DExCon-mNeonGreen-R11b

Plasmid

#179899

Purpose

Donor with homologous arms for Rab11b to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-59-DExCon-mTagBFP2-R25

Plasmid

#179900

Purpose

Donor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB25, member RAS oncogene family (RAB25 Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mTagBFP2

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_ANKRA2_ANK

Plasmid

#178466

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

ANKRA2_ANK (ANKRA2 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_DCUN1D1_PONY

Plasmid

#178473

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

DCUN1D1_PONY (DCUN1D1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_KLC1_TPR

Plasmid

#178477

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KLC1_TPR (KLC1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-AMOT p130∆PPxY1(L106P)

Plasmid

#166714

Purpose

expresses AMOT(L106P) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 L106P (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

changed leucine 106 to proline

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-AMOT p130∆PPxY1(A112K)

Plasmid

#166715

Purpose

expresses AMOT(A112K) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 A112K (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

changed alanine 112 to lysine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_03

Plasmid

#158104

Purpose

CaV1.2 5'UTR luciferase reporter

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR of CACNA1C (uc009zdu.1)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Orf6

Plasmid

#157726

Purpose

mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Orf6 (ORF6 SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp15

Plasmid

#157720

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp14

Plasmid

#157717

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp14 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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KCC4_HUMAN_D0

Plasmid

#79744

Purpose

This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC4 (SLC12A7 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KS6A1_HUMAN_D1

Plasmid

#79720

Purpose

This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KS6A1 (RPS6KA1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK10_HUMAN_D0

Plasmid

#79722

Purpose

This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK10 (STK10 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only