We narrowed to 18,031 results for: Mut

1. p-spTorA-GFP-H6C

   Plasmid

   #168517

   Purpose

   GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.

   Depositor

   Siegfried Musser

   Article

   Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

   Insert

   spTorA-GFP-H6C
   

   Use

   Tags

   6xHisC tag and spTorA (signal peptide of E. coli …

   Expression

   Bacterial

   Mutation

   The mut3 GFP variant with 5 additional mutations …

   Promoter

   araBAD

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG-MTOR-E1799K

   Plasmid

   #69009

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-E1799K (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Glutamate 1799 to Lysine

   Promoter

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-BG505.SOSIP-TM

   Plasmid

   #111844

   Purpose

   Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Use

   Tags

   CD5 leader sequence and TM helix from MHC class I

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT TO Myc ArA K162R

   Plasmid

   #59805

   Purpose

   Allows the integration of Myc ArA K162R in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora A (AURKA Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K162R, kinase mutant

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGM190

   Plasmid

   #69614

   Purpose

   inducible gene expression in Streptomyces

   Depositor

   Günther Muth

   Article

   Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

   Inserts

   aphII
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Use

   Streptomyces- e. coli shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ATG-1929

   Plasmid

   #108712

   Purpose

   CBR2opt in pF4Ag expression vector with T7 and CMV promoter

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   T7, CMV

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG27

   Plasmid

   #226742

   Purpose

   Expresses a reporter for a mitochondrial matrix protein

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   COX4 (COX4I1 Human)
   

   Use

   Lentiviral

   Tags

   GFP1-10

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-MTOR-T1977R

   Plasmid

   #69011

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-T1977R (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Threonine 1977 to Arginine

   Promoter

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1(+)- e18 LOF

   Plasmid

   #131729

   Purpose

   Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-4EBP1 F113A

    Plasmid

    #81122

    Purpose

    expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    4EBP1 F113A (Eif4ebp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F113A mutation

    Promoter

    CAG

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Promoter

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGV3341

    Plasmid

    #170279

    Purpose

    Expression of mutant HIV-2 RT p66 reverse transcriptase

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    HIV-2 RT p66 (mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-MTOR-F1888L

    Plasmid

    #69010

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-F1888L (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Phenylalanine 1888 to Leucine

    Promoter

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-FLEX-SaCas9-U6-sgGrin2a

    Plasmid

    #124850

    Purpose

    Mutagenesis of Grin2a

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2a (Grin2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

    Plasmid

    #124860

    Purpose

    Mutagenesis of Slc17a6

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a6 (Slc17a6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGV3340

    Plasmid

    #170278

    Purpose

    Expression of mutant HIV-2 RT p51 reverse transcriptase

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    HIV-2 RT p51 (mutant)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmcherry α-catenin M319G/R326E

    Plasmid

    #178648

    Purpose

    express α-catenin with M319G/R326E mutation

    Depositor

    Daniel Conway

    Article

    Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

    Insert

    α-catenin (CTNNA1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    M319G/R326E

    Promoter

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. cytosolic moPrP (3F4)

    Plasmid

    #1321

    Depositor

    Susan Lindquist

    Article

    Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

    Insert

    PrP (Prnp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N-term truncation (to make gene cytosolic), vario…

    Promoter

    Available Since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pRCPam

    Plasmid

    #195861

    Purpose

    Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.

    Depositor

    Gregory Phillips

    Article

    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

    Insert

    Amber Chromogenic Protein on pRCPam plasmid
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gln62X

    Promoter

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3-V5-PTPN14-PPxY

    Plasmid

    #61006

    Purpose

    Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 PPxY mutation (PTPN14 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    PPxY mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only