We narrowed to 10,053 results for: codon optimized

1. pLenti-VRER-PGK-Puro

   Plasmid

   #110856

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFC334

   Plasmid

   #87846

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   argB
   yA specific sgRNA
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   A. nidulans gpdA promoter with Hammerhead ribozym…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIV298

   Plasmid

   #204957

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTRdeltaGFPmirGE-DroshaT1.5.5

   Plasmid

   #61084

   Purpose

   pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

   Depositor

   Patrick Salmon

   Article

   Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

   Insert

   deltaGFP
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIV302

   Plasmid

   #204973

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   nat1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIV299

   Plasmid

   #204967

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and argB selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   argB
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMesh1*

   Plasmid

   #175591

   Purpose

   Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Mesh1 (Mesh1 Fly)
   

   Use

   Tags

   Fused with a flexible GS linker to cerulean

   Expression

   Bacterial

   Mutation

   Codon optimized for translation in E.coli. AGGAGG…

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-mios in pRK5

    Plasmid

    #154212

    Purpose

    N-terminal HA-tagged Mios cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    Mios
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    cDNA sequence has been codon optimized using huma…

    Promoter

    CMV

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMesh1

    Plasmid

    #175594

    Purpose

    Lac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    Mesh1 (Mesh1 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for translation in E.coli. AGGAGG…

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pESC-URA-hPOLISc

    Plasmid

    #60819

    Purpose

    to express human Pol iota recoded to yeast codons in yeast

    Depositor

    Youri Pavlov

    Article

    Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

    Insert

    POLI (POLI Human)
    

    Use

    Tags

    c-myc

    Expression

    Yeast

    Mutation

    optimized to yeast codon usage

    Promoter

    GAL1

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pscALPS-SIV-AGM-TAN1 vpr

    Plasmid

    #115819

    Purpose

    Encodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVAGM Vpr (TAN1)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCarCBA

    Plasmid

    #117754

    Purpose

    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCarCBAE

    Plasmid

    #117755

    Purpose

    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUB_SM-FLuc0_EMCV-Ren

    Plasmid

    #210590

    Purpose

    Expresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Inserts

    Spaghetti monster (FLAG)-nonoptimal FLuc
    Optimal Renilla luciferase
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    EMCV IRES and UBC human

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCarCBADE

    Plasmid

    #117757

    Purpose

    Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised CarCBA, Protein CarD, Ferredoxin CarE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    constitutive 35SPPDK promoter

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. human Alpha-synuclein

    Plasmid

    #51437

    Purpose

    human codon optimized Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pSMH1741

    Plasmid

    #160930

    Purpose

    Expression of soluble Sc octamer (H3)

    Depositor

    Stephen Harrison

    Inserts

    HHF1
    HTA1
    HTB1
    HHT1
    

    Use

    Tags

    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…

    Expression

    Mutation

    codon-optimized for E. coli -- see provided seque…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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