We narrowed to 6,714 results for: hwa

1. p2xp53_RE

   Plasmid

   #118053

   Purpose

   GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   2 copies of the p53 DNA binding motif
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p4xSMAD_RE

   Plasmid

   #118051

   Purpose

   GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   4 copies the SMAD DNA binding motif
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Expression

   Mammalian

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRc/CMV-Beta(4)Integrin

   Plasmid

   #16265

   Depositor

   Arthur Mercurio

   Article

   Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

   Insert

   Beta 4 Integrin (ITGB4 Human)
   

   Expression

   Mammalian

   Mutation

   lacked the 70- and 7-amino acid splice variants a…

   Available Since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

   Plasmid

   #83489

   Purpose

   cre-dependent PAK1-AID under EF1a promoter

   Depositor

   Dong Kong

   Article

   Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

   Insert

   PAK1-AID-ires-EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLIK-Neo-TGmiR-Luc

   Plasmid

   #25745

   Purpose

   Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Luciferase miR-shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIMVAd39T

   Plasmid

   #98303

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

   Expression

   Yeast

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1_VNp6-StefinA

   Plasmid

   #182404

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-stefin (CSTA Human)
   

   Tags

   Vesicle Nucleating peptide 6 (VNp6)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

   Plasmid

   #99221

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos3.1
   

   Expression

   Bacterial

   Mutation

   A69T for primed conversion applications

   Promoter

   T7

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

   Plasmid

   #98973

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 2 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98972

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(D10A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-FOXO3a T32A DBM (H212R)

    Plasmid

    #1793

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, H212R

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pCMV5-ratERK2-Q103A

    Plasmid

    #40816

    Depositor

    Natalie Ahn

    Article

    Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

    Insert

    MAPK1 (Mapk1 Rat)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Q103A

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

13. c-myc promoter (Frag B)

    Plasmid

    #16606

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Frag B) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    FragB (KpnI->AccI). The KpnI site is at -1194 …

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Tags

    mRuby3

    Expression

    Yeast

    Promoter

    pHis3

    Available Since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. c-myc promoter (Frag C)

    Plasmid

    #16607

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Frag C) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    FragC (AccI->PvuII). The AccI site is at -741 …

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pBeast-pT7-soxA

    Plasmid

    #190057

    Purpose

    Express the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    SoxA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-REPORT (PGK) 5xGRE-NanoLuc

    Plasmid

    #207170

    Purpose

    GAl4 response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

    Inserts

    NanoLuciferase
    d2eGFP
    5x GRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Promoter

    Gal4 response element minP and PGK

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. RSRC1

    Plasmid

    #155983

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    RSRC1 (RSRC1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ps226

    Plasmid

    #133557

    Purpose

    RNAi construct for knock-down of CDKN1A (Cip1, p21)

    Depositor

    David Livingston

    Article

    Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Retroviral

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. IKK2-EYFP

    Plasmid

    #111196

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

    Insert

    IKKbeta (IKBKB Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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