We narrowed to 31,369 results for: Eng

1. pAGM1479

   Plasmid

   #50291

   Depositor

   Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR, omega (Tobacco Mosaic Virus) + N terminal HIS tag (6x polyhistidine)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICH78141

   Plasmid

   #50293

   Depositor

   Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (Oryza sativa)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAGM5331

   Plasmid

   #50294

   Depositor

   Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR, omega (Tobacco Mosaic Virus) + nuclear localisation signal (Simian Virus 40)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pICSL30010

   Plasmid

   #50305

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS no stop codon, mEOS2 photoactivatable fluorescent protein (Lobophyllia hemprichii)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pICH44157

   Plasmid

   #50258

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter, RbcS2B (AT5g38420, A. thaliana)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICSL13002

   Plasmid

   #50266

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (0.4 kb ) + 5'UTR, 35s (Cauliflower Mosaic Virus)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pICH44222

   Plasmid

   #50287

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR CMV1 (Cucumber Mosaic Virus)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pICH44188

   Plasmid

   #50289

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR BSMV (Barley Stripe Mosaic Virus)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-CasRx-non-targeting (pEZ18)

   Plasmid

   #229049

   Purpose

   Cloning backbone for CasRx crRNA (U6 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-Cas13bt1-non-targeting (pJK2)

    Plasmid

    #229054

    Purpose

    Cloning backbone for Cas13bt1 crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. phU1-Cas13bt1-non-targeting (pEZ25)

    Plasmid

    #229055

    Purpose

    Cloning backbone for Cas13bt1 crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-RanCas13b-non-targeting (pJK6)

    Plasmid

    #229056

    Purpose

    Cloning backbone for RanCas13b crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. phU1-RanCas13b-non-targeting (pEZ45)

    Plasmid

    #229057

    Purpose

    Cloning backbone for RanCas13b crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SJZ6-HIS5

    Plasmid

    #228259

    Purpose

    Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    his5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 S.c.

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. His-MBP-FL-Efg1

    Plasmid

    #216395

    Purpose

    Expresses MBP-tagged full length Efg1

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-FL
    

    Use

    Tags

    6xHis tags-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDonor_Tn[GFP]

    Plasmid

    #213903

    Purpose

    Mariner transposon expressing mNeonGreen with kan selection casette

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDonor_Tn[RFP]

    Plasmid

    #213904

    Purpose

    Mariner transposon expressing mCherry with cam selection casette

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with cam resistance and mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgRNA1 _8xCTS-ECFP-pA

    Plasmid

    #200243

    Purpose

    Mammalian transfections; 8xCTS-ECFP reporter construct 1

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    sgRNA1_8xCTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgRNA1 _1xCTS-ECFP-pA

    Plasmid

    #200237

    Purpose

    Mammalian transfections; 1xCTS-ECFP reporter construct 1

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    sgRNA1_1xCTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA5 _1xCTS-ECFP-pA

    Plasmid

    #200241

    Purpose

    Mammalian transfections; 1xCTS-ECFP reporter construct 5

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    sgRNA5_1xCTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. sgRNA3 _1xCTS-ECFP-pA

    Plasmid

    #200239

    Purpose

    Mammalian transfections; 1xCTS-ECFP reporter construct 3

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    sgRNA3_1xCTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. gRNA4 _8xCTS-ECFP-pA

    Plasmid

    #200246

    Purpose

    Mammalian transfections; 8xCTS-ECFP reporter construct 4

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    sgRNA4_8xCTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. sgRNA4 _1xCTS-ECFP-pA

    Plasmid

    #200240

    Purpose

    Mammalian transfections; 1xCTS-ECFP reporter construct 4

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    sgRNA4_1xCTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. sgRNA2 _1xCTS-ECFP-pA

    Plasmid

    #200238

    Purpose

    Mammalian transfections; 1xCTS-ECFP reporter construct 2

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    sgRNA2_1xCTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. bpNLS-DddIA

    Plasmid

    #187847

    Purpose

    expresses the bpNLS-linked DddIA in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    bpNLS-DddIA-bpNLS-p2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. B7QAT8

    Plasmid

    #163244

    Purpose

    Inducible expression of UniProt identifier B7QAT8

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    B7QAT8
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. A0A0D6UPR3

    Plasmid

    #163247

    Purpose

    Inducible expression of UniProt identifier A0A0D6UPR3

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    A0A0D6UPR3
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Q0K750

    Plasmid

    #163248

    Purpose

    Inducible expression of UniProt identifier Q0K750

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    Q0K750
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. W6W585

    Plasmid

    #163250

    Purpose

    Inducible expression of UniProt identifier W6W585

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    W6W585
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. B8LUI6

    Plasmid

    #163251

    Purpose

    Inducible expression of UniProt identifier B8LUI6

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    B8LUI6
    

    Use

    Tags

    10xHis and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge