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JD602: pMVP (L3-L2) APEX2 + WPRE

Plasmid

#121798

Purpose

pMVP L3-L2 entry plasmid, contains APEX2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

APEX2 + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC601: pMVP (L3-L2) eYFP + polyA

Plasmid

#121769

Purpose

pMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC801: pMVP (L3-L2) eGFP + WPRE

Plasmid

#121771

Purpose

pMVP L3-L2 entry plasmid, contains eGFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LC901: pMVP (L3-L2) mCherry + WPRE

Plasmid

#121773

Purpose

pMVP L3-L2 entry plasmid, contains mCherry + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LD001: pMVP (L3-L2) eYFP + WPRE

Plasmid

#121777

Purpose

pMVP L3-L2 entry plasmid, contains eYFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KG901: pMVP (L3-L2) HA epitope tag + polyA

Plasmid

#121749

Purpose

pMVP L3-L2 entry plasmid, contains HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LC701: pMVP (L3-L2) eCFP + polyA

Plasmid

#121767

Purpose

pMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IO102: pMVP (R4-R3) eGFP open

Plasmid

#121731

Purpose

pMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HJ601: pMVP (R4-R3) mCherry open

Plasmid

#121733

Purpose

pMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IO601: pMVP (R4-R3) eCFP open

Plasmid

#121735

Purpose

pMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IO401: pMVP (R4-R3) eYFP open

Plasmid

#121737

Purpose

pMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KH305: pMVP (R4-R3) βgal open

Plasmid

#121739

Purpose

pMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

βgal (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LF801: pMVP (L1-R5) cTnT promoter

Plasmid

#121696

Purpose

pMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

cTnT promoter (Cardiomyocyte specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IH001: pMVP (L5-L4) HA epitope tag

Plasmid

#121699

Purpose

pMVP L5-L4 entry plasmid, contains HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IH201: pMVP (L5-L4) 3x HA epitope tag

Plasmid

#121700

Purpose

pMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

3x HA epitope tag (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IX203: pMVP (L5-L4) V5 epitope tag

Plasmid

#121705

Purpose

pMVP L5-L4 entry plasmid, contains V5 epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

V5 epitope tag (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KU801: pMVP (L5-L4) mCherry

Plasmid

#121709

Purpose

pMVP L5-L4 entry plasmid, contains mCherry for 4-component MultiSite Gateway Pro assembly. Allows N-term mCherry fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KU901: pMVP (L5-L4) eCFP

Plasmid

#121711

Purpose

pMVP L5-L4 entry plasmid, contains eCFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eCFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KP401: pMVP (L5-L4) eCFP-P2A

Plasmid

#121712

Purpose

pMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KV001: pMVP (L5-L4) eYFP

Plasmid

#121713

Purpose

pMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
gh162

Plasmid

#106815

Purpose

expression of gRNA targeting TMTC3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

TMTC3 (TMTC3 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pME GFP-DCX

Plasmid

#105962

Purpose

entry clone for gateway cloning

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NP_835365.1 (DCX Human)

Tags

6xHis-thrombin cleavage site and EGFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA27_no stop

Plasmid

#83860

Purpose

Gateway donor vector for IAA27 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA27 (PAP2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA8_no stop

Plasmid

#83874

Purpose

Gateway donor vector for IAA8 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 8 (IAA8 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA5_no stop

Plasmid

#83868

Purpose

Gateway donor vector for IAA5 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA5 (IAA5 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA3_no stop

Plasmid

#83863

Purpose

Gateway donor vector for IAA3 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA3 (SHY2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA10_no stop

Plasmid

#83848

Purpose

Gateway donor vector for IAA10 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA4_no stop

Plasmid

#83866

Purpose

Gateway donor vector for IAA4 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA4 (ATAUX2-11 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA28_no stop

Plasmid

#83861

Purpose

Gateway donor vector for IAA28 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

NP_568478.1 (IAA28 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-IAA15_no stop

Plasmid

#83853

Purpose

Gateway donor vector for IAA15 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 15 (IAA15 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB425

Plasmid

#74819

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFOSGE_COF4F04

Plasmid

#71030

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sin3A (Sin3A Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B10

Plasmid

#70840

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sce (Sce Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2C08

Plasmid

#70911

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf5 (Taf5 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2C05

Plasmid

#70891

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc2 (Orc2 Fly)

Use

Gateway entry vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00584

Plasmid

#39668

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG15216 (CG15216 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00513

Plasmid

#39614

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7960 (Bro Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00715

Plasmid

#39743

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG31224 (CG31224 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00610

Plasmid

#39687

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG31169 (CG43342 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00691

Plasmid

#39731

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG14441 (CG14441 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00608

Plasmid

#39686

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG30077 (blos1 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00577

Plasmid

#39661

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4575 (CG4575 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00528

Plasmid

#39626

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG31510 (CG31510 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00525

Plasmid

#39625

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8089 (CG8089 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00512

Plasmid

#39613

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7786 (CG7786 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00749

Plasmid

#39755

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG15455 (RunxB Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00748

Plasmid

#39754

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4820 (CG4820 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00631

Plasmid

#39701

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11405 (Atf3 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00625

Plasmid

#39697

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12236 (CG12236 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00492

Plasmid

#39596

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG31835 (CG31835 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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