We narrowed to 27,033 results for: Cat

1. pLentiPGK Puro DEST JNKKTR AE Clover

   Plasmid

   #90240

   Purpose

   Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (AE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC/CMV-JAK1P1084A-VSV

   Plasmid

   #139358

   Purpose

   Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

   Depositor

   Sandra Pellegrini

   Article

   Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

   Insert

   JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   P1084A

   Promoter

   CMV

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBiex PKC - Peptide 8 mCit mCer

   Plasmid

   #83563

   Purpose

   Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PRKCA (PRKCA Human)
   

   Use

   Tags

   10 nm ER/K Helix, FLAG purification tag, TEV Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV hSyn1 CaRhAC T2A tDimer

   Plasmid

   #101722

   Purpose

   - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Inserts

   CatRhAC
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E497K,C569D

   Promoter

   human Synapsin1 promotor and ribosomal skip seque…

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. XE257 pCDNA3.1(zeo)-hDsh3-truncDEP

   Plasmid

   #16752

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3-trunc before DEP (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncated before DEP

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. L-CRISPR-CTN (MLL-ctrl)

   Plasmid

   #69215

   Purpose

   Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV promoter
   P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3_shRNA resistant PKA-Cα-mEGFP

   Plasmid

   #114143

   Purpose

   Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Caveolin3DGV

   Plasmid

   #67886

   Purpose

   Fluorescently tagged Caveolin-3 truncation mutant for expression in mammalian cells

   Depositor

   Rob Parton

   Article

   Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

   Insert

   Cav3 (Cav3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the first 53 amino acids of Caveolin3

   Promoter

   CMV

   Available since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

9. DF-UAS-sspa-GCaMP6f

   Plasmid

   #67569

   Purpose

   Zebrafish expression of a short photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   sspa-GCaMP6f
   

   Use

   Zebrafish transgenesis (see :a spinal opsin contr…

   Tags

   Expression

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

10. shYES1 # 1

    Plasmid

    #42546

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

11. bu6-sgCebpa_v2-mU6-sgCebpb_v2-hU6-sgCebpd_v2

    Plasmid

    #177258

    Purpose

    Expresses Cebpa_v2 (bU6), Cebpb_v2 (mU6), Cebpd_v2 (hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_v2/sgCebpb_v2/sgCebpd_v2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6/mU6/hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-REX-GECO0.9

    Plasmid

    #61247

    Purpose

    Expresses REX-GECO0.9 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

    Insert

    REX-GECO0.9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1: P60R, V61W, R6…

    Promoter

    CMV

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

13. XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ

    Plasmid

    #16754

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3-trunc before PDZ (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncated before PDZ

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPN098

    Plasmid

    #91625

    Purpose

    Express sgRNA targeting human HCN1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    HCN1 (HCN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPN099

    Plasmid

    #91626

    Purpose

    Express sgRNA targeting human HCN1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    HCN1 (HCN1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. DF-UAS-spa-GCaMP6f

    Plasmid

    #67568

    Purpose

    Zebrafish expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Tags

    HIS-tag

    Expression

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET28b Me31B-3Myc

    Plasmid

    #113001

    Purpose

    For protein expression and purification of Myc-tagged full-length Drosophila me31B

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length me31B (me31B Fly)
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPN234

    Plasmid

    #91591

    Purpose

    Express sgRNA targeting human CYFIP1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPN262

    Plasmid

    #91607

    Purpose

    Express sgRNA targeting human EPHX2

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    EPHX2 (EPHX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 49535-sgFMR1_E17A

    Plasmid

    #157782

    Purpose

    Expresses a sgRNA targeting stop codon of human FMR1 gene

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1 (FMR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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