We narrowed to 7,819 results for: Lif

1. pcDNA3-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123329

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-AKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

   Plasmid

   #123330

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-AKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKH3-TRIM28-C65A/C68A

   Plasmid

   #92199

   Purpose

   Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   Tif1b (TRIM28 Human, Synthetic)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Cysteines 65 and 68 to Alanines.

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAW-Nb127D01-mCherry

   Plasmid

   #171573

   Purpose

   Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-mCherry (CXCR2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   fly actin5C promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tet-O-FUW-EGFP-P2A-mCherry

   Plasmid

   #203868

   Purpose

   Tet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

   Plasmid

   #114377

   Purpose

   expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP and Kv2.1C-linker-TlcnC

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRXIB-mRuby3-CERT(PHD)

   Plasmid

   #221762

   Purpose

   Stable expression of mRuby3-CERT(PHD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   CERT (CERT1 Human)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   amino acids 1–116

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

   Plasmid

   #180010

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA 3.1 mitoAURKA

   Plasmid

   #157750

   Purpose

   Expression of AURKA targeted to mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Aurora A without the first 30AA + MTS of CoxVIII

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Plxnd1-Fc-His

    Plasmid

    #72132

    Purpose

    Expresses the extracellular region of the PlexinD1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnd1 (Plxnd1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. L1cam-Fc-His

    Plasmid

    #72083

    Purpose

    Expresses the extracellular region of the L1CAM protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    L1cam (L1cam Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR EGFR-SLP76-TagBFP

    Plasmid

    #188630

    Purpose

    Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    SLP76-TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

    Plasmid

    #180011

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

    Plasmid

    #180012

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ACE2-Fc

    Plasmid

    #164222

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    ACE2 extracellular domain (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-mCherry-p2A-full length LIC1

    Plasmid

    #74601

    Purpose

    expresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-cT3G-cERP2-WT1

    Plasmid

    #192906

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of WT1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    WT1 (WT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-CLK1

    Plasmid

    #174088

    Purpose

    Inducibly expresses Myc-CLK1 in mammalian cells with the Tet-on system

    Depositor

    Katie Ullman, Wesley Sundquist

    Article

    Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

    Insert

    CLK1 (CLK1 Human)
    

    Use

    Lentiviral

    Tags

    Myc-GSSS

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-cT3G-cERP2-PPARG

    Plasmid

    #192912

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of PPARG

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    PPARG (PPARG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICSL11060

    Plasmid

    #68264

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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