We narrowed to 5,133 results for: puc

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pLacZa-GFP

Plasmid

#86440

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

LacZalpha-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

LacZa-sfGFP

Promoter

Plac

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAK005

Plasmid

#128218

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 5

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 5

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAK003

Plasmid

#128216

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 3

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 3

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAK004

Plasmid

#128217

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 4

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 4

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK006

Plasmid

#128219

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 6

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 6

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK002

Plasmid

#128215

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 2

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 2

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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TC872

Plasmid

#164895

Purpose

TRE::nCre, inducible nCre expression when co-express with rtTA

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

nCre

Expression

Worm

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_N_allele_400

Plasmid

#161767

Purpose

Donor vector for endogenous tagging of human FUS at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCHW00016

Plasmid

#222447

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

p65AD-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0248

Plasmid

#162313

Purpose

Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-sfGFP-HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGGF003

Plasmid

#48844

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::D-AlanineR::tMAS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCJ155

Plasmid

#175054

Purpose

psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Promoter

rbcLXS

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGGA004

Plasmid

#48815

Purpose

Provides the 35S promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

35S promoter

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site removed by substit…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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HNRNPA1_Halo_C_allele

Plasmid

#178123

Purpose

Donor vector for endogenous tagging of human HNRNPA1 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (HNRNPA1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAK008

Plasmid

#128225

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 2

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 2

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK009

Plasmid

#128226

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 3

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 3

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ADAM10_Halo_C_allele

Plasmid

#178151

Purpose

Donor vector for endogenous tagging of human ADAM10 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ADAM10 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAD01 S. cerevisiae telomerase RNA KBS

Plasmid

#61890

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces cerevisiae KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pART (pT-2)

Plasmid

#62708

Purpose

Mammalian expression of tdTomato from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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AP575-1

Plasmid

#71277

Purpose

Expresses 3Xflag::tagRFP::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagRFP

Tags

3xFlag and myc

Expression

Bacterial

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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