Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 3,145 results for: N-EGFP

Showing: 2881 - 2900 of 3145 results

  1. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid
    #223108
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223108-plasmid-map-sequence-id-447631

    Enlarge

  2. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid
    #223109
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223109-plasmid-map-sequence-id-447628

    Enlarge

  6. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

    Enlarge

  11. cmamxA2-GFP

    Plasmid
    #107197
    Purpose
    Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted
    Depositor
    Ursula Rescher, Volker Gerke
    Article
    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8
    Insert
    cmannexin A2 (ANXA2 Human)
    Use
    Tags
    Green Fluorescent Protein
    Expression
    Mammalian
    Mutation
    all type II Ca2+ binding sites deleted ( D161A, E…
    Promoter
    CMV
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107197-plasmid-map-sequence-id-209669

    Enlarge

  12. pSpot-Tag-Lamin

    Plasmid
    #117062
    Purpose
    For mammalian expression of lamin B1 with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    Lamin B1 (LMNB1 Human)
    Use
    Tags
    Spot-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117062-plasmid-map-sequence-id-224387

    Enlarge

  13. pSpot-Tag-Actin

    Plasmid
    #117060
    Purpose
    For mammalian expression of beta-actin with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    actin beta (ACTB Human)
    Use
    Tags
    Spot-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117060-plasmid-map-sequence-id-224388

    Enlarge

  16. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

    Enlarge

  17. MADR pDonor smFP-Myc (dark) WPRE

    Plasmid
    #130987
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-Myc WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    Myc - 10 total Myc tags
    Expression
    Mutation
    "dark" variant with GGG fluorophore ver…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    130987-plasmid-map-sequence-id-305000

    Enlarge

Showing: 2881 - 2900 of 3145 results