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pLKO.1 shMTH1-2

Plasmid

#21298

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-2 (NUDT1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pCfB2513

Plasmid

#67543

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP4L

Plasmid

#104507

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Nov. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC578

Plasmid

#174366

Purpose

dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

dCas9 fused to SYNZIP18

Expression

Bacterial

Promoter

J23150

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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BII-ChPtinGW

Plasmid

#133377

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pILGFP4H

Plasmid

#104503

Purpose

Used to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SaGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4J

Plasmid

#104505

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4N

Plasmid

#104509

Purpose

Used to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SbGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4R

Plasmid

#104513

Purpose

Used to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SuGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBM13h-sd4U

Plasmid

#179690

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N wildtype

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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EZ-L498

Plasmid

#131771

Purpose

PPGK1_FUSN_Citrine _PixE_TCYC1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PPGK1_FUSN_Citrine _PixE_TCYC1

Use

Integration into his3 locus

Expression

Yeast

Promoter

PGK

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFREE2-eSpCas9-sgRNA

Plasmid

#179580

Purpose

Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

eSpCas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB2400

Plasmid

#67552

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0058

Plasmid

#117539

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

sgRNA (SaCas9)

Expression

Plant

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCO489

Plasmid

#139732

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_AtuMas:NnLuz:trbcSE9

Use

Luciferase

Expression

Plant

Promoter

AtuMAS

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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PABE-2

Plasmid

#115623

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB2198

Plasmid

#67554

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3

Plasmid

#112658

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

sfGFP-Glo3

Tags

sfGFP

Expression

Yeast

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2228

Plasmid

#67540

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pDS66

Plasmid

#48364

Purpose

3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)

Use

Cre/Lox; Knockout in t. brucei

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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