We narrowed to 7,632 results for: ef1a
-
Plasmid#216099PurposeKnockout, EF1a-driven Cas12a (Cas only)DepositorInsertCas12a [EnAs]
UseCRISPR and Lentiviral; Assembled vectorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-Hes1
Plasmid#36983DepositorAvailable SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)-DD-W/Bla
Plasmid#201560PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DDExpressionMammalianPromoterEF1a and pIND(SP1)Available SinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A K107/113R-3xFlag_IRES-GFP
Plasmid#198392PurposeRPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationubiquitination site double mutant (K107/113R)PromoterEF1AAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoNMM:mCherry
Plasmid#111878PurposeThis plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.DepositorInsertCapsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
UseCapsaspora owczarzakiTagsmCherryPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AA065
Plasmid#215965PurposeFragmid fragment: (Pol 2 promoter) human EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEF1a_v1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-BLST-Cas9-VPR
Plasmid#115147PurposeSafe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassetteDepositorInsertBlastR-P2A-Cas9-VPR
UseCRISPRTagsSV40NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS7107
Plasmid#89051PurposeExpresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9DepositorInsertsNm crRNA
Nm tracrRNA
hNmCas9
UseCRISPRTagsHA tag, NLS, and SV40 NLSPromoterEF1a and human U6Available SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTJK281 (miR-513a-5p mut OE in pWCC52)
Plasmid#254386PurposeExpress human miR-513a-5p mutantDepositorInsertmiR-513a-5p mutant (containing 2 base mutations in mature miR-513a-5p sequence) (MIR513A1 Human)
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceApril 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTJK282 (miR-513a-3p mut OE in pWCC52)
Plasmid#254387PurposeExpress human miR-513a-3p mutantDepositorInsertmiR-513a-3p mutant (containing 2 base mutations in mature miR-513a-3p sequence) (MIR513A1 Human)
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceApril 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pANC56(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-KKYL-IRES-EGFP)
Plasmid#248355PurposeLentiviral vector that can express hAqp1_ER export based sensor for TEVp in mammalian cells. Expressed off of EF1a promoter with EGFP markerDepositorInsertFLAG7 hAqp1-GSG-(TEVcs-GSG)3x-KKYL
UseLentiviralTags3x TEV site, KKYL and FLAG (internal in Aqp1)PromoterEF-1aAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPD684 EnAsCas12a-BFP
Plasmid#249157PurposeOverexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains Cp36 recombination site.DepositorInsertEnAsCas12a-TagBFP
UseCRISPR; Cp36 donor dnaExpressionMammalianPromoterEF1aAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPD686 A2UCOE-EnAsCas12a-BFP
Plasmid#249159PurposeOverexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.DepositorInsertA2UCOE-EnAsCas12a-TagBFP
UseCRISPR; Cp36 donor dnaExpressionMammalianPromoterEF1aAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdLbCas12a-tagBFP-NLS-3xFlag
Plasmid#236385PurposeOverexpression of HyperdLbCas12a in human cellsDepositorInsertHyperdLbCas12a
UseCRISPR and LentiviralMutationD156R, D235R, E292R, D350RAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbCas12a-EGFP-NLS-3xFlag
Plasmid#236364PurposeOverexpression of dLbCas12a in human cellsDepositorInsertdLbCas12a
UseCRISPR and LentiviralMutationWTAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdLbCas12a-EGFP-NLS-3xFlag
Plasmid#236367PurposeOverexpression of HyperdLbCas12a in human cellsDepositorInsertHyperdLbCas12a
UseCRISPR and LentiviralMutationD156R, D235R, E292R, D350RAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbCas12aRVRR-EGFP-NLS-3xFlag
Plasmid#236365PurposeOverexpression of dLbCas12aRVRR in human cellsDepositorInsertdLbCas12aRVRR
UseCRISPR and LentiviralMutationG532R, K538V, Y542R, K595RAvailable SinceDec. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag
Plasmid#236376PurposeOverexpression of HyperdFnCas12a in human cellsDepositorInsertHyperdFnCas12a
UseCRISPR and LentiviralMutationE184R, T279R, D340R, D409RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag
Plasmid#236375PurposeOverexpression of dFnCas12a in human cellsDepositorInsertdFnCas12a
UseCRISPR and LentiviralMutationWTAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag
Plasmid#236363PurposeOverexpression of endAsCas12a in human cellsDepositorInsertendAsCas12a
UseCRISPR and LentiviralMutationE174R, S542R, K548RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only