We narrowed to 11,996 results for: pcDNA

1. pcDNA3-FBL6 delta6

   Plasmid

   #19925

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   FBL6 delta 6 (FBXL6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   delta 6

   Promoter

   Available since

   May 7, 2009

   Availability

   Academic Institutions and Nonprofits only

2. HA-USPL1-C236A-pcDNA3.1

   Plasmid

   #85764

   Purpose

   mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   catalytic inactive mutant C236A

   Promoter

   CMV

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-USPL1-C236S-pcDNA3.1

   Plasmid

   #85763

   Purpose

   mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   catalytic inactive mutant C236S

   Promoter

   CMV

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)Gαi1-91-NLuc

   Plasmid

   #207148

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI1 (GNAI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)K

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-h-mmPtch1-FL-TagBFP

   Plasmid

   #120921

   Purpose

   Expresses BFP tagged Ptch1

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-mCherry-N

   Plasmid

   #210271

   Purpose

   N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)_Granulin 2+linker3

   Plasmid

   #176920

   Purpose

   express Granulin 2 with linker 3 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Granulin 2+linker3 (GRN Human)
   

   Use

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

   Plasmid

   #159132

   Purpose

   Expresses BACH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   BACH1 (BACH1 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Y11F

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA4.TO-K8a-2xCSTREP

   Plasmid

   #136158

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8a

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   K8a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. T7-p73DD L371P-pcDNA3

    Plasmid

    #23049

    Depositor

    William Kaelin

    Article

    Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

    Insert

    tumor protein p73 (TP73 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    truncated cDNA encodes truncated protein (aa 327-…

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Lyn-sapphireAKAR

    Plasmid

    #118466

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-sapphireAKAR
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA-FRT-FAK20

    Plasmid

    #59120

    Purpose

    FAK fret sensor with 20 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-ROR1-V5/HIS

    Plasmid

    #205607

    Purpose

    Expression of human ROR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROR1 receptor tyrosine kinase (ROR1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Flag-YOD1

    Plasmid

    #49784

    Purpose

    Express YOD1 with a N-terminal Flag-tag in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

    Insert

    YOD1 (YOD1 Human)
    

    Use

    Tags

    Flag-tag

    Expression

    Mutation

    WT

    Promoter

    Available since

    Dec. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1+_LN-HA-AGO2-WT

    Plasmid

    #92009

    Purpose

    Expresses Lambda-N-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-TTF-1-HDD

    Plasmid

    #52063

    Purpose

    It encodes a mutant of TTF-1 in which the homeodomain is deleted

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    Thyroid Transcription Factor 1 (NKX2-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Homeodomain deleted

    Promoter

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-CHRFAM7A-mGFP

    Plasmid

    #62633

    Purpose

    mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    CHRFAM7A (CHRFAM7A Human)
    

    Use

    Tags

    mEGFP inserted in M3-M4 loop

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5FRT/TO-p97wt-mycStrep

    Plasmid

    #31837

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Use

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA4.TO-ORF39-2xCSTREP

    Plasmid

    #136200

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF39

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF39
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY117 (pcDNA3.1-huMb3Cpf1)

    Plasmid

    #92293

    Purpose

    Expression of humanized Mb3Cpf1

    Depositor

    Feng Zhang

    Insert

    huMb3Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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