We narrowed to 7,725 results for: pcr
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Bacterial strain#230034PurposeFluorescently labelled (mCherry) E. coliDepositorBacterial resistanceNoneAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only
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pR26-CMVconst
Plasmid#127373PurposeAllows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingPromoterCMV enhancer/promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKan-PCUP1-9myc-AID*(N)
Plasmid#99527PurposeN-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW1354
Plasmid#69490PurposeGly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology armsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
UseCRISPR; Template to generate cassettes for -media…Available sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)
Plasmid#44095DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)
Plasmid#44083DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…Available sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ML17
Bacterial strain#61912PurposeC43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. glnA gene deleted.DepositorBacterial resistanceNoneAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
punc-119cR
Plasmid#21596DepositorInsertunc-119:mCherry (unc-119 Nematode)
UseAdds unc-119 marker to amp resistant plasmidsTagsmCherryExpressionWormMutationN/AAvailable sinceAug. 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GBP-kanMX6
Plasmid#89067Purposegenomic GBP epitope C-terminal tagging in S. pombeDepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsGBPExpressionYeastAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-tagged NRP1 (human)
Plasmid#213068PurposeExpress myc-NRP1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertneuropilin 1 (NRP1 Human)
TagsMycExpressionMammalianMutationN-terminal myc tag was introduced by a three-step…PromoterT7Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RF2
Bacterial strain#62070PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deletedDepositorBacterial resistanceNoneAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Pepper-fused sgRNA
Plasmid#217520PurposeExpresses Pepper-fused sgRNA in mammalian cells with U6 promoterDepositorInsertPepper-fused sgRNA for spCas9
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCY 3110-01
Plasmid#36236DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmEos2
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-mEos2-kanMXAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
STAgR_Neo
Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsPromoterU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
STAgR_tdTomato
Plasmid#102993PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsPromoterU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR26-CMVconst
Plasmid#127373PurposeAllows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingPromoterCMV enhancer/promoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YFP(1-158)-beta-1 in pcDNAI/Amp
Plasmid#54468PurposeAn amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYFP (1-158)/beta-1 (GNB1 Aequorea victoria, Human)
TagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-1 was amplified via PCR, which removed an i…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-9myc-AID*(N)
Plasmid#99527PurposeN-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMYC BRGI aa597-1407
Plasmid#19146DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
TagsMycExpressionMammalianMutationhuman BRG1 aa 597-1407 (PCR product)Available sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJW1354
Plasmid#69490PurposeGly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology armsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
UseCRISPR; Template to generate cassettes for -media…Available sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only