We narrowed to 1,516 results for: SIR

1. pDONR223-TAF5-FL_WobbleMut

   Plasmid

   #209053

   Purpose

   Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 full length
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-TAF5-∆ex8_WobbleMut

   Plasmid

   #209054

   Purpose

   Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 deltaexon-8
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-SENP2 NPm

   Plasmid

   #126593

   Purpose

   Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell lines

   Depositor

   Joanna Morris

   Article

   Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

   Insert

   SENP2 (SENP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-65 and L329A/L331A in t…

   Promoter

   CMV

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCMV-Blast mLST8-Flag Mut1

   Plasmid

   #124916

   Purpose

   For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L mutant

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MLST8 (MLST8 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Insert ORF was sirently mutated to be resistent t…

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCMV-Blast mLST8-Flag Mut2

   Plasmid

   #124917

   Purpose

   For stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutant

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MLST8 (MLST8 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Insert ORF was sirently mutated to be resistent t…

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLAP-BUBR1-3A

   Plasmid

   #114054

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1-3A (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance), BUBR1-S670A…

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLAP-BUBR1-3D

   Plasmid

   #114055

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1-3D (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance), BUBR1-S670D…

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5` CC: Puro – loxP – GFP-C

   Plasmid

   #219563

   Purpose

   5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   hPGK-promoter_PuroR_2A_loxP_GFP-C
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC119-gRNA

   Plasmid

   #52255

   Purpose

   Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Arabidopsis U6 polymerase III promoter

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR-53BP1 1-1972 WT

    Plasmid

    #53446

    Purpose

    Recombinational donor/master vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    siRNA resistant and mutations A128V, T720A, A1347…

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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