We narrowed to 5,632 results for: chia

1. AAV-CREB

   Plasmid

   #68550

   Purpose

   AAV expression of CREB

   Depositor

   Eric Nestler

   Article

   Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

   Insert

   CREB
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAPDH-Cre-HYG

   Plasmid

   #231510

   Purpose

   Expresses Cre recombinase in Acanthamoeba

   Depositor

   Yeonchul Hong

   Insert

   Cre recombinase
   

   Use

   Unspecified; Acanthamoeba castellanii neff strain

   Tags

   EGFP and SV40 NLS

   Expression

   Mutation

   Promoter

   Acanthamoeba GAPDH

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pM9-BetaGal-8C

   Plasmid

   #172489

   Purpose

   Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   M9-BetaGal-8C
   

   Use

   Tags

   6xHis and M9 nuclear localization sequence (from …

   Expression

   Bacterial

   Mutation

   BetaGal has two cysteine mutations (C77A, C1022S)…

   Promoter

   T7

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CamKIIa:smFP-TVA-RGV

   Plasmid

   #202594

   Purpose

   Expresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.

   Depositor

   Oleksandr Shcheglovitov

   Article

   Chiola et al Mol Psychiatry. 2021 Feb 8. pii: 10.1038

   Inserts

   smFP
   TVA
   RGV
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJBEI-6411

   Plasmid

   #47050

   Purpose

   BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
   

   Use

   Synthetic Biology; Bglbrick

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. NgΔIQ FUNgDIQGW

   Plasmid

   #92232

   Purpose

   Lentiviral expression of human Ng with deletion mutation

   Depositor

   Weifeng Xu

   Article

   Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

   Insert

   Ng (NRGN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta IQ

   Promoter

   hUbC

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORTMAGE-1

   Plasmid

   #72680

   Purpose

   Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   lower strength RBS before mutL E32K allele than i…

   Promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pPICZ-pre-pro-alphaf-Btl2

   Plasmid

   #117664

   Purpose

   Study secretion efficiency of Btl2

   Depositor

   Benjamin Glick, Pau Ferrer

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-pro-alphaf-Btl2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the lipase Btl2 together wit…

   Promoter

   pAOX1

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 4LNDDE488Q-pCDNA3.1(+)

   Plasmid

   #172228

   Purpose

   Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

   Depositor

   Joshua Rosenthal

   Article

   Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain E488Q
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   changed the Glutamic acid 488 in the Deaminase Do…

   Promoter

   CMV

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMJ923

    Plasmid

    #78313

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-mCherry-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2CG2-QUAS5x:GFPNLS-SV40pA

    Plasmid

    #155122

    Purpose

    Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUAS5x:GFPNLS
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    QUAS5x-E1b

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. BtuF

    Plasmid

    #92209

    Purpose

    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter

    Depositor

    Douglas Rees

    Article

    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99

    Insert

    BtuF (btuF )
    

    Use

    Tags

    6x His Tag and PelB Signal Sequence

    Expression

    Bacterial

    Mutation

    BtuF signal sequence removed and uses the PelB si…

    Promoter

    T7 Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Per2:iLuc

    Plasmid

    #160293

    Purpose

    target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.

    Depositor

    Joseph Takahashi

    Article

    Shan et al Neuron. 2020 Aug 4. pii: S0896-6273(20)3

    Insert

    Per2LUC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    no promotor

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCarCBADE_ProB(I69E)A

    Plasmid

    #117756

    Purpose

    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-SIRT2-Flag

    Plasmid

    #102624

    Purpose

    Expresses full length SIRT2 isoform 1 in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

    Plasmid

    #155125

    Purpose

    Tol2 vector containing Cre-inducible QFGal4-switch

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4-switch
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Ubi

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbE1k-3

    Plasmid

    #71722

    Purpose

    tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase (QDPR Human)
    pterin-4a-carbinolamine dehydratase (PCBD1 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI syn iGluu

    Plasmid

    #106122

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR S72T variant
    

    Use

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Ser 72 to Thr

    Promoter

    synapsin

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-mCREB

    Plasmid

    #68551

    Purpose

    AAV expression of mCREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    mCREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S133A

    Promoter

    CMV

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pC009 LshCas13a locus with targeting spacer

    Plasmid

    #91900

    Purpose

    LshCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with targeting spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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