We narrowed to 4,217 results for: erf

1. pHR Ef1a-dCas9-BFP-KRAB

   Plasmid

   #217304

   Purpose

   lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

   Depositor

   Luke Gilbert

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Ef1alpha

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExp-sfGFP

   Plasmid

   #112573

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis, Strep II, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-EF1alpha-dCas9-KRAB_Hygro

   Plasmid

   #192663

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH385-GST-TEV-SUP35

   Plasmid

   #29744

   Depositor

   Tobias von der Haar

   Insert

   SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   wild type

   Promoter

   Available since

   Aug. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH278-GST-TEV-SUP45

   Plasmid

   #29743

   Depositor

   Tobias von der Haar

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   wild type

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRL-TK CXCR4 1P

   Plasmid

   #11309

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

   Insert

   1 perfect bind site for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB CAG-R-3xSV40pA-R-td-sfGFP

   Plasmid

   #177150

   Purpose

   CAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Tandem dimer superfolder GFP
   

   Use

   Dre / rox ; piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3 CXCR4 1P

   Plasmid

   #11310

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

   Insert

   1 perfect binding site for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Pp-luc

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-loxP-3xpA-H2BsfGFP-tdTomato - opt GS PQR Var3 P2A -APGST E

   Plasmid

   #113847

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by P2A (-APGST linker)

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-P2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3 CXCR4 2P

    Plasmid

    #11311

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    2 perfect binding sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Pp-luc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. U6+27-16pf

    Plasmid

    #21976

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    U6 miR-16 perfect
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pTH340-SUP45(1-242)-GalBD

    Plasmid

    #29740

    Depositor

    Tobias von der Haar

    Insert

    SUP45 (SUP45 Budding Yeast)
    

    Use

    Tags

    Gal Activation domain and Myc

    Expression

    Yeast

    Mutation

    Codons 1-242 of the yeast SUP45 gene only

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3 CXCR4 3P

    Plasmid

    #11312

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    3 perfect binding sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Pp-luc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C105m

    Plasmid

    #121008

    Purpose

    MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    sfYFP-AAV ssrA (has bbsI)
    

    Use

    Synthetic Biology

    Tags

    AAV degradation tag for ssrA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. C106m

    Plasmid

    #121009

    Purpose

    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    sfYFP-DAS ssrA (has bbsI)
    

    Use

    Synthetic Biology

    Tags

    DAS degradation tag for ssrA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH339-SUP45(1-138)-GalBD

    Plasmid

    #29739

    Depositor

    Tobias von der Haar

    Insert

    SUP45 (SUP45 Budding Yeast)
    

    Use

    Tags

    Gal Activation domain and Myc

    Expression

    Yeast

    Mutation

    Codons 1-138 of the yeast SUP45 gene only

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. U6+27-20pf

    Plasmid

    #21978

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    U6 miR-20 perfect
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

18. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid

    #192664

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, D839A, H840A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. XClone_Cas9_KRAB_T2A_BFP

    Plasmid

    #141208

    Purpose

    Tunable and temporal expression control of dCas9

    Depositor

    Rory Johnson

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOpen-HSlig3

    Plasmid

    #165574

    Purpose

    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    DNA Ligase III
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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