We narrowed to 39,931 results for: lat

1. pGEX-4T1-Claspin-FL-wt

   Plasmid

   #31621

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

   Insert

   Claspin (CLSPN Human)
   

   Use

   Tags

   Flag, GST, and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pET-DUET-GST-eIF4G1-His6, eIF4E

   Plasmid

   #37232

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Inserts

   TIF4631 (TIF4631 Budding Yeast)
   CDC33 (CDC33 Budding Yeast)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pG23A

   Plasmid

   #102884

   Purpose

   Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
   

   Use

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRXIP-GFP-STX17TMΔC

   Plasmid

   #221737

   Purpose

   Stable expression of GFP-STX17TMΔC in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   STX17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-228 and 227-302

   Promoter

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-MCPIP1(1-555)

   Plasmid

   #90105

   Purpose

   Mammalian expression of Flag-MCPIP1(1-555)

   Depositor

   Mingui Fu

   Article

   Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

   Insert

   MCPIP1 (ZC3H12A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mRIP3 GFP D161N

   Plasmid

   #41383

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP3 (Ripk3 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Kinase Domain Mutant (D161N)

   Promoter

   CMV

   Available Since

   Dec. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

8. psiCHECK2-miR-34 MT

   Plasmid

   #78259

   Purpose

   psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   mutated miR-34 target site
   

   Use

   Luciferase; Microrna activity

   Tags

   Expression

   Mammalian

   Mutation

   nucleotides 2-5 and 10-11 changed from CCGT to GG…

   Promoter

   Available Since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lentiviral CaMKKbeta

   Plasmid

   #33322

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   human PGK

   Available Since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

    Plasmid

    #191627

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-Cdk9 T186A

    Plasmid

    #28103

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Tags

    3x HA

    Expression

    Bacterial and Mammalian

    Mutation

    Contains T186A mutation in Cdk9

    Promoter

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TF-bio-His

    Plasmid

    #52184

    Purpose

    Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TF (TF Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSilencer2.1-U6-4JC1RR

    Plasmid

    #190847

    Purpose

    For mammalian expression of 4JC1RR, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    4JC1RR (A Dual-Specificity aptamer targeting OGT and GFP)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pSilencer2.1-U6-NL1F50

    Plasmid

    #190831

    Purpose

    For mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    NL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pJZC42

    Plasmid

    #66564

    Purpose

    sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1XPP7
    PCP-VP64 IRES mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMJB1-RPS22B-Cl

    Plasmid

    #160431

    Purpose

    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Insert

    RPS22B (RPS22B Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Removed introns, shuffled codons to change nucleo…

    Promoter

    GAL1 and S. cerevisiae GAL1

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-MCPIP1(delta ZF)

    Plasmid

    #90110

    Purpose

    Mammalian expression of Flag-MCPIP1(delta ZF)

    Depositor

    Mingui Fu

    Article

    Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

    Insert

    MCPIP1 (ZC3H12A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K317G, S318G

    Promoter

    CMV

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBbdCas9S(-10TC)_Psyn-sgRNA500

    Plasmid

    #149583

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBpuro-EGFP-eDHFR(69K6)

    Plasmid

    #178850

    Purpose

    Mammalian expression of EGFP-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    EGFP-eDHFR(69K6)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-mCherry-TSC2-NLS

    Plasmid

    #192441

    Purpose

    Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2-NLS (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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