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We narrowed to 3,237 results for: sam

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Showing: 2901 - 2920 of 3237 results

  12. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid
    #160734
    Purpose
    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160734-plasmid-map-sequence-id-318595

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  13. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

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  14. SUPV3L1-HA G207V

    Plasmid
    #232349
    Purpose
    Expresses human SUPV3L1/SUV3 (G207V) in mammalian cells with HA tag
    Depositor
    Samantha Lewis
    Insert
    SUPV3L1 (SUPV3L1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G207V
    Promoter
    CMV
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232349-plasmid-map-sequence-id-462147

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  15. pBG-ttLbUV2

    Plasmid
    #231386
    Purpose
    LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.
    Depositor
    Qi-Jun Chen
    Article
    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:
    Insert
    ttLbCas12a Ultra V2
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D156R and E795L
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    231386-plasmid-map-sequence-id-461803

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  16. pSL0284 (pQCascade_entry)

    Plasmid
    #130635
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130635-plasmid-map-sequence-id-255022

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  17. pQFmcs-2H12.D11-scarREF

    Plasmid
    #221184
    Purpose
    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    None (same as cdGreen2) and PQ5 (cumate-inducible)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221184-plasmid-map-sequence-id-437178

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Showing: 2901 - 2920 of 3237 results