We narrowed to 7,945 results for: Lif
-
Plasmid#188626PurposeHuman EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensorDepositorInsertEGFR (EGFR Synthetic, Human)
UseLentiviralTagsCD3ε-FusionRedExpressionMammalianPromoterSFFVAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-AKAR4
Plasmid#181957PurposeGenetically encoded FRET-based PKA activity reporter fused to AKAP79.DepositorInsertAKAP79-AKAR4 (AKAP5 Human)
TagsAKAP79, Cerulean, HIS tag, and cpVenus(E172)ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
ACE2
Plasmid#164219PurposeExpresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection markerDepositorInsertACE2 (ACE2 Human)
UseLentiviralMutationsilent mutation to remove internal EcoRI site (GA…PromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ALK-V5/HIS
Plasmid#200115PurposeExpression of human ALK receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TCF21
Plasmid#192894PurposeBarcoded piggybac transposon vector with Dox-inducible expression of TCF21DepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cSrc
Plasmid#214233PurposeBacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; HE1A:BFP -2
Plasmid#180012PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV AURKA-mTurq2
Plasmid#157767PurposeExpression of AuroraA kinase fused to mTurquoise2 under CMV promoterDepositorAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-ING2(PHD)
Plasmid#221751PurposeStable expression of GFP-ING2(PHD) in mammalian cellsDepositorInsertING2 (ING2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtwo ING2(PlantHD) domains (amino acids 190–280)Available SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJR100
Plasmid#187240PurposeLentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expressionDepositorInsertLentiviral sgRNA vector for Perturb-seq with mU6 promoter
UseLentiviralAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn LAMP1-msGFP (JV012)
Plasmid#179728PurposeLentiviral expression of LAMP1-msGFP for use as a lysosomal markerDepositorInsertLAMP1 (Lamp1 Mouse)
UseLentiviralAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-PyronicSF/pCMV-myc-nuc
Plasmid#124814PurposeHighly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.DepositorInsertPyronicSF
TagsNuclear Localization SignalExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTP434
Plasmid#104167PurposeBacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
Tags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation3x Cysteines located at bps 493-495, 520-522, and…Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-HA-His
Plasmid#171567PurposeBacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)DepositorInsertNbVHH05-HA-His (UBE2J1 Human)
TagsHA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-FBXL10
Plasmid#126542PurposeExpresses GFP-tagged FBXL10 in mammalian cellsDepositorAvailable SinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -0
Plasmid#180007PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -1
Plasmid#180008PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TAF4B
Plasmid#192927PurposeBarcoded piggybac transposon vector with Dox-inducible expression of TAF4BDepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-GCaMP7f-CCR5TC
Plasmid#216534PurposeEncodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
TagsGCaMP7fExpressionMammalianPromoterCAGAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAW-Nb127D01-GFP
Plasmid#171571PurposeExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cellsDepositorAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only