We narrowed to 11,157 results for: RIK

1. pDGE502

   Plasmid

   #153275

   Purpose

   M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-mPar6a K19A

   Plasmid

   #86149

   Purpose

   FLAG-mPar6a K19A - Does not bind to aPKC

   Depositor

   Senthil Muthuswamy

   Article

   Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

   Insert

   Par6 (Pard6a Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lysine to Alanine mutation K19A

   Promoter

   LTR

   Available since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

3. CAG-Cas9-T2A-EGFP-ires-puro

   Plasmid

   #78311

   Purpose

   Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

   Depositor

   Timo Otonkoski

   Article

   Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

   Insert

   WT SpCas9-T2A-EGFP-ires-puromycin resistance
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. RN3P-KDM5B-FLAG

   Plasmid

   #86398

   Purpose

   FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

   Depositor

   Wei Xie

   Article

   Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

   Insert

   KDM5B (Kdm5b Mouse)
   

   Use

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Lac

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA6-N-3XFLAG-Gsk3b

   Plasmid

   #123592

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   glycogen synthase kinase 3 beta (Gsk3b Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCXN2KS-Dsup

   Plasmid

   #90019

   Purpose

   To establish stable line expressing Dsup in constitutive manner

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA6-C-Flag-GK5_v2

   Plasmid

   #134282

   Purpose

   Expresses Flag-tagged GK5 (isoform 2) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse Gk5 isoform 2; 534 amino acids

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF0126

   Plasmid

   #141530

   Purpose

   Lentiviral vector for overexpressing the CXXC1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CXXC1 (CXXC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMECP2-GFP

   Plasmid

   #181904

   Purpose

   Fluorescently tagged MECP2, GFP

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   MECP2 (Mecp2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p(HA)HIF1alpha(401delta603)R27G

    Plasmid

    #52215

    Purpose

    mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and R27G mutation

    Depositor

    Eric Huang

    Article

    Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

    Insert

    HIF1alpha (401delta603) R27G (HIF1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 401-603 deleted, removes the oxygen-dependent …

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLNCX2-NGN2-IRES-GFP-T2A-Sox11

    Plasmid

    #90213

    Purpose

    To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

    Insert

    NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1 _3xHA-Ago2_RNAmut

    Plasmid

    #73539

    Purpose

    Inducible lentiviral expression of Ago2_RNAmut

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Small RNA binding mutant PAZ domain Y311A and F31…

    Promoter

    TRE promoter, Tet ON

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1 _3xHA-Ago2_CATmut

    Plasmid

    #73540

    Purpose

    Inducible lentiviral expression of Ago2_CATmut

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Catalytic mutant PIWI domain D670A

    Promoter

    TRE promoter, Tet ON

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-ZIP(RK)

    Plasmid

    #27136

    Depositor

    Ron Vale

    Article

    Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

    Insert

    CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    eGFP and mCherry

    Expression

    Mammalian

    Mutation

    Arginines in the SH2 domains mutated to lysines (…

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMBP-parallel1-GK5_v2

    Plasmid

    #134288

    Purpose

    Expresses GK5 (isoform 2) in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    mouse Gk5 isoform 2; 534 amino acids

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pv6_MECP2_MBD-R106W

    Plasmid

    #179408

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MECP2_MBD(R106W) (Mecp2 Mouse)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    MBD R106W

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcGFP1-N1-Dsup-deltaC

    Plasmid

    #90023

    Purpose

    To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal region

    Promoter

    CMV

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cntn5.1-AP-His

    Plasmid

    #71943

    Purpose

    Expresses the extracellular region of the Contactin 5, isoform 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn5.1 (Cntn5 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-ZIP(FF)

    Plasmid

    #27135

    Depositor

    Ron Vale

    Article

    Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

    Insert

    CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    eGFP and mCherry

    Expression

    Mammalian

    Mutation

    Tyrosines in ITAMS 1-3 mutated to phenylalanines …

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cntn5.1-Fc-His

    Plasmid

    #72069

    Purpose

    Expresses the extracellular region of the Contactin 5, isoform 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn5.1 (Cntn5 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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