We narrowed to 6,485 results for: plasmid dna

1. pLV.U6.gDNMT1.Cas9-T2A-GFP

   Plasmid

   #170362

   Purpose

   Plasmid used for Crispr/cas9 based disruption of human DNMT1.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL.175

   Plasmid

   #184996

   Purpose

   Express -Eco1 HEK3 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   HEK3 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ATP9A-luciferase

   Plasmid

   #113354

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   ATP9A-enhancer (ATP9A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCL.177

   Plasmid

   #184998

   Purpose

   Express -Eco1 EMX1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   EMX1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL.180

   Plasmid

   #185001

   Purpose

   Express -Eco1 AAVS1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   AAVS1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK2-miR-34 MT

   Plasmid

   #78259

   Purpose

   psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   mutated miR-34 target site
   

   Use

   Luciferase; Microrna activity

   Tags

   Expression

   Mammalian

   Mutation

   nucleotides 2-5 and 10-11 changed from CCGT to GG…

   Promoter

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE4254

   Plasmid

   #89050

   Purpose

   Expresses St chimeric gRNA and human codon-optimized StCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   St chimeric gRNA
   hStCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1-FHC-POLQ-S1977P

   Plasmid

   #64876

   Purpose

   lentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   S1977P, mimicking the chaos1 mutation

   Promoter

   EF1alpha

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. SunTagCACTA1g2-22aa-TET1cd

   Plasmid

   #106437

   Purpose

   SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

   Depositor

   Steven Jacobsen

   Article

   Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFETCh_KMT2B

    Plasmid

    #101070

    Purpose

    Donor vector for 3' FLAG tag of human KMT2B

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    KMT2B homology arms (KMT2B Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZJQIBEBT003

    Plasmid

    #140053

    Purpose

    The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

    Depositor

    Jia Zhang

    Article

    Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

    Insert

    eMutS-L157C-G233C-CBM-EGFP
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    L157C, G233C

    Promoter

    T7

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jean-Loup Faulon, Jerome Bonnet

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV.U6.gLacZ.Cas9-T2A-GFP

    Plasmid

    #170361

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFETCh_ARID4B

    Plasmid

    #101072

    Purpose

    Donor vector for 3' FLAG tag of human ARID4B

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ARID4B homology arms (ARID4B Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFETCh_RERE

    Plasmid

    #101069

    Purpose

    Donor vector for 3' FLAG tag of human RERE

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RERE homology arms (RERE Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458_RERE_1

    Plasmid

    #101073

    Purpose

    Encodes gRNA for 3' target of human RERE

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against RERE (RERE Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold

    Plasmid

    #226933

    Purpose

    Encoding Nme2ABE8e and sgRNA cassette on a single AAV

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    TadA8e, nNme2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold

    Plasmid

    #226935

    Purpose

    Encoding SaKKHABE8e and sgRNA cassette on a single AAV

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    TadA8e, nSaKKHCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AA201

    Plasmid

    #216026

    Purpose

    Fragmid fragment: (Cas protein) firefly luciferase

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Fluc_v1.1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge