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pENTR-R4R3-mRuby-C

Plasmid

#113755

Purpose

Gateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2

Use

CRISPR; Gateway entry vector

Tags

mRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmRuby-C

Plasmid

#113757

Purpose

Gateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

3xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SERA3-bio-His

Plasmid

#50815

Purpose

Expresses enzymatically monobiotinylated full length SERA3

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA3

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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SERA4-bio-His

Plasmid

#50816

Purpose

Expresses enzymatically monobiotinylated full length SERA4

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA4

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PF13_0125-bio-His

Plasmid

#50808

Purpose

Expresses enzymatically monobiotinylated full length PF13_0125

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF13_0125

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PF14_0044-bio-His

Plasmid

#50809

Purpose

Expresses enzymatically monobiotinylated full length PF14_0044

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF14_0044

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PFA0135w-bio-His

Plasmid

#50810

Purpose

Expresses enzymatically monobiotinylated full length PFA0135w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFA0135w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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SERA2-bio-His

Plasmid

#50814

Purpose

Expresses enzymatically monobiotinylated full length SERA2

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA2

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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MSP3.5-bio-His

Plasmid

#50803

Purpose

Expresses enzymatically monobiotinylated full length MSP3.5

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSP3.5

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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MSP8-bio-His

Plasmid

#50807

Purpose

Expresses enzymatically monobiotinylated full length MSP8 ectodomain

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSP8

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-zEPLIN

Plasmid

#40957

Depositor

Elizabeth Luna, Scott Maul

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN (lima1 Zebrafish)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
4xHRE_CMV53-sfGFP-CMV_dsRed Exp

Plasmid

#117402

Purpose

sfGFP expressed from hypoxia-inducible CMV53 promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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4xHRE_miniTK-sfGFP-CMV_dsRed Exp

Plasmid

#117404

Purpose

sfGFP expressed from hypoxia-inducible miniTK promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch12_62418577-gRNA-rev

Plasmid

#81217

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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ARF17 pGreenII0229

Plasmid

#12070

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

ARF17 (ARF17 Mustard Weed)

Use

Plant transformation via agrobacterium

Expression

Plant

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA

Plasmid

#87587

Purpose

PV8 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCALNL-eGFP-Esp3I

Plasmid

#81226

Purpose

Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

Esp31-Neo-Esp31 deletion cassette upstream of EGFP

Expression

Mammalian

Promoter

pCBa

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAG148 SGCD 3'UTR

Plasmid

#12006

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N13 3'UTR (SGCD Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG192 PIK3CG 3'UTR

Plasmid

#12019

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N23 3'UTR (PIK3CG Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR H1 L5-L4

Plasmid

#62102

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-eBE-S1

Plasmid

#101092

Purpose

Expresses eBE-S1 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 L1-L4

Plasmid

#62112

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-for1

Plasmid

#81210

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCALNL_Ch10

Plasmid

#81224

Purpose

pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Expression

Mammalian

Promoter

pCBa

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHU6_gRNA_Ch10-rev1

Plasmid

#81208

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG216 NEK6 3'UTR mut

Plasmid

#12046

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N17 3'UTR mut (NEK6 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR H1 L5-L2

Plasmid

#62103

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG178 RGS7 3'UTR mut

Plasmid

#12036

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N11 3'UTR mut (RGS7 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-B6

Plasmid

#31710

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-19b

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
ARF17 pGreenII0129

Plasmid

#12080

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

ARF17 (ARF17 Mustard Weed)

Use

Plant transformation via agrobacterium

Expression

Plant

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
pAG195 TNKS2 3'UTR

Plasmid

#11999

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

TNKS2 3'UTR (TNKS2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG226 SFRS12 3'UTR mut

Plasmid

#12045

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

SFRS12 3'UTR mut (SREK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG220 CASC2 3'UTR mut

Plasmid

#12050

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N21 3'UTR mutant (CASC2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG183 SRPK2 3'UTR mut

Plasmid

#12041

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N16 3'UTR mut (SRPK2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pAG218 NID1 3'UTR mut

Plasmid

#12048

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N19 3'UTR mut (NID1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pAG81 ZAP3 3'UTR mut

Plasmid

#12057

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N3 3'UTR mut (YLPM1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG187 RIPK4 3'UTR

Plasmid

#12063

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N20 3'UTR (RIPK4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p-mCherry

Plasmid

#249067

Purpose

Expresses nucleus-localized mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

mCherry

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_AAVS1D

Plasmid

#183340

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1D

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_AAVS1C

Plasmid

#183339

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1C

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_NTC4

Plasmid

#183336

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC4

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_NTC3

Plasmid

#183335

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC3

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_NTC2

Plasmid

#183334

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC2

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_NTC1

Plasmid

#183333

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC1

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0574 CAG-ADAR1-p150

Plasmid

#247268

Purpose

Expresses human ADAR1-p150 from the CAG promoter

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

ADAR1-p150 (ADAR Human)

Expression

Mammalian

Mutation

WT

Promoter

CAG

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_BRD4

Plasmid

#183331

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRD4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRD4

Use

Lentiviral

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS

Plasmid

#244836

Purpose

Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Gai-mTagBFP-3BEY-P2A-Gid-4LTB-GFP

Plasmid

#240137

Purpose

Mammalian plasmid expression both components of synDrops separated using P2A

Depositor

Liam Holt

Article

Shu et al PRX Life. 2024 Sep;2(3):033001. doi: 10.

Inserts

Gai-mTagBFP-3BEY
Gid-4LTB-GFP

Expression

Bacterial and Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SpRY-Tad-CBE(CCG3)

Plasmid

#235083

Purpose

Expresses SpRY-Tad-CBE(CCG3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCG3)-SpRY Cas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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