We narrowed to 166,544 results for: Gene

1. GTH-r

   Plasmid

   #81106

   Purpose

   Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   mCherry
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330-MRPsg1

   Plasmid

   #87189

   Purpose

   disruption of human RMRP gene via CRISPR-Cas9, guide 1

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   human MRP RNA CRISPR guide #1 (RMRP Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-P17

   Plasmid

   #120028

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mEos4b
   

   Use

   Pcr template

   Tags

   mEos4b

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMaCTag-Z06

   Plasmid

   #120049

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   sfGFP
   

   Use

   Pcr template

   Tags

   sfGFP

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMaCTag-17

   Plasmid

   #119996

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mEos4b

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mEos4b
   

   Use

   Pcr template

   Tags

   mEos4b

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBW300ParsR-Amp32T

   Plasmid

   #78652

   Purpose

   pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
   

   Use

   Synthetic Biology

   Promoter

   see insert

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

7. px330-MRPsg2

   Plasmid

   #87190

   Purpose

   disruption of human RMRP gene via CRISPR-Cas9, guide 2

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   human MRP RNA CRISPR guide #2 (RMRP Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pISH-Emx2

   Plasmid

   #74346

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx2. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

   Insert

   empty spiracles homeobox 2 (Emx2 Mouse)
   

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMaCTag-34

   Plasmid

   #119998

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   3xFLAG
   

   Use

   Pcr template

   Tags

   3xFLAG

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid

    #133340

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-P01

    Plasmid

    #120012

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    moxBFP
    

    Use

    Pcr template

    Tags

    moxBFP

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. B108-Otx2-IRES-Cre

    Plasmid

    #73993

    Purpose

    B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    B108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)
    

    Expression

    Mammalian

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMaCTag-27

    Plasmid

    #120006

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    1xHA
    

    Use

    Pcr template

    Tags

    1xHA

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUDP003

    Plasmid

    #101164

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

    Plasmid

    #133334

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-P08

    Plasmid

    #120019

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mClover3
    

    Use

    Pcr template

    Tags

    mClover3

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMaCTag-Z03

    Plasmid

    #120046

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    moxCerulean3
    

    Use

    Pcr template

    Tags

    moxCerulean3

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMaCTag-28

    Plasmid

    #120007

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xHA
    

    Use

    Pcr template

    Tags

    3xHA

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMaCTag-Z14

    Plasmid

    #120057

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeptune2.5
    

    Use

    Pcr template

    Tags

    mNeptune2.5

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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