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pSR1058

Plasmid

#208743

Purpose

Characterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PHbs

Use

Synthetic Biology

Expression

Bacterial

Promoter

PHbs

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET11_Ec_ORF209

Plasmid

#69029

Purpose

Expresses E.coli ORF209/ADPRase in bacterial cells

Depositor

Maurice Bessman, Sandra Gabelli

Article

Gabelli et al Nat Struct Biol. 2001 May;8(5):467-72.

Insert

ORF209/ADPRase

Tags

-

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only
pDM291-tetR'-tup11Δ70

Plasmid

#41028

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetR'-tup11Δ70

Expression

Yeast

Mutation

tup11 starts at aa70

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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LSL

Plasmid

#66590

Purpose

Lox-STOP-Lox reporter

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a- mYFP-kanMX6

Plasmid

#105147

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-kanMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTXG

Plasmid

#52280

Purpose

Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Type

Empty backbone

Tags

GyrA-intein-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pNY-351

Plasmid

#231048

Purpose

Used for staining GFP

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to goat Fc

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCM130

Plasmid

#45828

Depositor

Mary Lidstrom

Article

Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

Type

Empty backbone

Use

Low-background broad-host-range promoter-probe ve…

Promoter

none

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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p7XC3H

Plasmid

#47065

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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iGFP

Plasmid

#66589

Purpose

inverted GFP reporter

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

GFP

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pGGC026

Plasmid

#48831

Purpose

Provides 3xmCherry as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

3xmCherry

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Level 1A

Plasmid

#102701

Purpose

Mobius Assembly Level 1 Acceptor Vector A

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:spisPink:rrnBT1-T7TE

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGGG002

Plasmid

#48851

Purpose

Provides an H-A adapter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

H-A adapter - short

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEP-EGFPin

Plasmid

#60961

Purpose

PCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

eGFP-Kana_I-SceI

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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Tg(krt4:EGFP-Mmu.Rpl10a)

Plasmid

#128839

Purpose

RNA isolation from keratinocytes via TRAP

Depositor

Anna Huttenlocher

Article

Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

Insert

EGFP-L10a

Use

Zebrafish expression

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGGG001

Plasmid

#48850

Purpose

Provides an F-H adapter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

F-H adapter - short

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB000

Plasmid

#48857

Purpose

Empty entry vector for creating GreenGate N-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Entry vector

Expression

Mammalian

Promoter

tight TRE

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBXC3H

Plasmid

#47068

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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