We narrowed to 6,096 results for: pcDNA 3.1

1. pcDNA3-sACE2v3-Fc(IgA1)

   Plasmid

   #145158

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 3

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sACE2v5-Fc(IgA1)

   Plasmid

   #145160

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 5

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sACE2v2(732)

   Plasmid

   #154099

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available Since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-sACE2v4-Fc(IgA1)

   Plasmid

   #145159

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 4

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mPC1_4119-eGFP

   Plasmid

   #108394

   Purpose

   Genomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   variant ID rs13468401 missense variant R4237Q

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-mPC1_4148-3HA

   Plasmid

   #108398

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Tags

   3HA

   Expression

   Mammalian

   Mutation

   variant ID rs13468401 missense variant R4237Q

   Promoter

   CMV

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-mPC1_4148-eGFP

   Plasmid

   #108397

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   variant ID rs13468401 missense variant R4237Q

   Promoter

   CMV

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-hPC1_MTS_V4146I-eGFP

   Plasmid

   #108405

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-hPC1_MTS_L4137P-eGFP

   Plasmid

   #108403

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-hPC1_MTS-eGFP

    Plasmid

    #108402

    Purpose

    Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Mouse)
    

    Tags

    3xHA and eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-dCas9-FLp300

    Plasmid

    #61356

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-sACE2v2.4(732)-8h

    Plasmid

    #154103

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-G…

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-sACE2v2(732)-8h

    Plasmid

    #154102

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-sACE2v6-Fc(IgA1)

    Plasmid

    #145161

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-sPDGFRalpha-Fc-Y206S

    Plasmid

    #136439

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524) …

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-sPDGFRalpha-Fc-V242K

    Plasmid

    #136440

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524) …

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-Flag-SPRTN-wt

    Plasmid

    #110214

    Purpose

    Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

    Depositor

    Kristijan Ramadan, Ivana Marinovic-Terzic

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Expression

    Mammalian

    Mutation

    P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3_N-DYK_MTHFD1-NLS

    Plasmid

    #133023

    Purpose

    NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

    Depositor

    Stefan Kubicek

    Article

    Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

    Insert

    MTHFD1 (MTHFD1 Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3_N-DYK_MTHFD1-NES

    Plasmid

    #133022

    Purpose

    NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

    Depositor

    Stefan Kubicek

    Article

    Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

    Insert

    MTHFD1 (MTHFD1 Human)
    

    Tags

    NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-PARL-D319N-FLAG C-t

    Plasmid

    #191957

    Purpose

    Expression of Presenilin Associated Rhomboid Like (PARL) D319N mutant

    Depositor

    Luca Scorrano

    Insert

    PARL D319N (PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D319N

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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