We narrowed to 7,661 results for: ski

1. BtA2-3A

   Plasmid

   #238984

   Purpose

   In vitro transcription of mutant bovine arrestin-2 long isoform

   Depositor

   Vsevolod Gurevich

   Article

   Celver et al J Biol Chem. 2002 Mar 15;277(11):9043-8.

   Insert

   arrestin-2 long (ARRB2 Bovine)
   

   Use

   In vitro transcription

   Mutation

   I386A,V387A,F388A

   Promoter

   SP6

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLS-CMV-mP-luc

   Plasmid

   #213506

   Purpose

   Positive control vector for luciferase lentivirus-based enhancer assay

   Depositor

   Nadav Ahituv

   Article

   Deng et al Science. 2024 May 24;384(6698):eadh0559.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS413-TEF1pr-CFAPorig

   Plasmid

   #221092

   Purpose

   FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS413-TEF1pr-NFAPorig

   Plasmid

   #221091

   Purpose

   FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS415-TEF1pr-NFAPorig

   Plasmid

   #221086

   Purpose

   FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS415-TEF1pr-CFAPorig

   Plasmid

   #221087

   Purpose

   FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS19

   Plasmid

   #229475

   Purpose

   Expresses RssB with E135A, E136A, & E137A mutation

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (E135A, E136A, E137A)
   

   Expression

   Bacterial

   Mutation

   E135A, E136A, E137A

   Promoter

   T7

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS21

   Plasmid

   #229476

   Purpose

   Expresses RssB with E135A, E136A, & E137A mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (E135A, E136A, E137A)
   

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   E135A, E136A, E137A

   Promoter

   T7

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVF18

   Plasmid

   #229456

   Purpose

   Expresses IraD (aa17-130) with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   IraD (aa 17-130 only)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 17-130 only

   Promoter

   T7

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM201

    Plasmid

    #227660

    Purpose

    pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Tags

    3x-FLAG-HRV3C-

    Expression

    Yeast

    Mutation

    (amino acids 121-131) VTENKIF mutated to AAAAAAA

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TCT-Universal plasmid

    Plasmid

    #221852

    Purpose

    A universal plasmid for expressing any protein with TetraCysteine motif

    Depositor

    Artur Krężel

    Article

    Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

    Insert

    Tetra Cysteine tag
    

    Expression

    Bacterial

    Mutation

    No

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRAR119

    Plasmid

    #185051

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L34 coding region, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L34
    

    Expression

    Bacterial

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. RT-5

    Plasmid

    #220442

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (puf-3 Nematode)
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. RT-4

    Plasmid

    #220443

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (PUF3 Budding Yeast)
    

    Tags

    3xHA

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-MBP-TEVpCS-ADGRF2 CTF-FLAG

    Plasmid

    #217695

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF2 (ADGRF2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-429

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-MBP-TEVpCS-ADGRE4 CTF-FLAG

    Plasmid

    #217692

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE4 (ADGRE4P Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-173

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-MBP-TEVpCS-ADGRA1-FLAG

    Plasmid

    #217678

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA1 (ADGRA1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3'-BirA*-FLAG minimal CMV pCDNA5

    Plasmid

    #212047

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5'-BirA*-FLAG minimal CMV pCDNA5

    Plasmid

    #212046

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 5'-3xFLAG GFP minimal CMV pCDNA5

    Plasmid

    #212086

    Purpose

    LR vector for integration of GFP into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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