Addgene

We narrowed to 881 results for: tes/1000

Showing: 421 - 450 of 881 results

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ATG9A-mCherry

Plasmid

#197393

Purpose

Expresses ATG9A in mammalian cells

Depositor

Juan Bonifacino

Article

De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

Insert

ATG9A (ATG9A Human)

Use

Tags

mCh

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW-APPSwe-T2A-mCherry

Plasmid

#190804

Purpose

Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

Depositor

Ronald Hart

Article

Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

Insert

APP (APP Human)

Use

Lentiviral

Tags

Expression

Mutation

Swedish (KM670/671NL)

Promoter

Ubi C

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-DN (S25N)

Plasmid

#101046

Purpose

Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGImC_V5-CSNK1A1

Plasmid

#92014

Purpose

Expresses V5-CSNK1A1, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Px461-Cas9n-Trp53-sgRNA-beta

Plasmid

#88847

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Px461-Cas9n-Trp53-sgRNA-alpha

Plasmid

#88846

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-GJA1-S373A

Plasmid

#52879

Purpose

Encodes human Connexin 43 resistant to siRNA with Serine to Alanine mutation at 373

Depositor

Robin Shaw

Article

Smyth et al Traffic. 2014 Mar 10. doi: 10.1111/tra.1

Insert

GJA1 (GJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Serine 373 mutated to Alalnine

Promoter

CMV

Available since

May 12, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

Plasmid

#159128

Purpose

expresses KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

KEAP1 (KEAP1 Human)

Use

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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His10-CD3z-SNAPf-ybbR (XSB507)

Plasmid

#78508

Purpose

Construct for expressing CD3z-SNAPf-ybbR in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

CD3z (Cytoplasmic domain) (CD247 Human)

Use

Tags

His10 and SNAPf-ybbR

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTag-RFP-C-h-Rab11a

Plasmid

#79806

Purpose

Express TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Use

Tags

TagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 proHB-EGF CS

Plasmid

#11601

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Use

Tags

His and myc

Expression

Mammalian

Mutation

Constitutively secreted HB-EGF: C-terminal amino …

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-GATA2

Plasmid

#125028

Purpose

doxycycline-inducible expression of human GATA2 in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

GATA binding protein 2 (GATA2 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCIneoGFP-BMI1

Plasmid

#128328

Purpose

Mammalian expression vector of GFP-tagged human BMI1

Depositor

Yutaka Hata

Article

Hossain et al Mol Cell Biol. 2018 Aug 15;38(17). pii:

Insert

BMI1 (BMI1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXSN-c-JUN

Plasmid

#102758

Purpose

Expression of c-Jun

Depositor

Jin Chen

Article

Song et al Cancer Res. 2014 May 1;74(9):2444-54. do

Insert

c-Jun (JUN Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-WT

Plasmid

#91978

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMG046 pETARA-CK1α-His

Plasmid

#154074

Purpose

Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Tags

GST and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-RNF51

Plasmid

#126546

Purpose

Expresses GFP-tagged RNF51 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF51 (BMI1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

LTR

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES2-DsRed-MST-Kv2.1

Plasmid

#131707

Purpose

Expresses Kv2.1in mammalian cells

Depositor

Jennifer Kearney

Article

Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

Insert

KCNB1 (KCNB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-CAD

Plasmid

#73566

Purpose

Soluble red fluorescent activator of Orai1 channels

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)

Use

Tags

6x His and mCherry

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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plenti-UBC- IRF4-3xHA-pGK-PUR

Plasmid

#107386

Purpose

Lentiviral expression of IRF4-3xHA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

UBC

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(S387A)

Plasmid

#220462

Purpose

Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to alanine

Promoter

CMV

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pssGFP-hCRT

Plasmid

#80022

Purpose

Mammalian expression of signal peptide-EGFP-humanCRT

Depositor

Chinten James Lim

Article

Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi

Insert

CRT (CALR Human)

Use

Tags

EGFP and signal peptide

Expression

Mammalian

Mutation

signal peptide 17aa added before GFP-CRT, will ta…

Promoter

CMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-WT-ATPIF1

Plasmid

#85403

Purpose

Retroviral vector expressing wild type human ATPIF1

Depositor

David Sabatini

Article

Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

Insert

ATPIF1 (ATP5IF1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Wildtype

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
ePB-PURO-FLAG-METTL3-APPA

Plasmid

#160253

Purpose

For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

tight TRE promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-CAD

Plasmid

#73567

Purpose

Soluble activator of Orai1 channels

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

CAD (STIM1 342-448) (STIM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-R438E

Plasmid

#91984

Purpose

Expresses FLAG-HA-AGO2 (R438E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

R438E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-K525E

Plasmid

#91985

Purpose

Expresses FLAG-HA-AGO2 (K525E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

K525E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pF713-pEGFP-N1-Hs-FL-METTL20 wt

Plasmid

#85114

Purpose

transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length METTL20 (METTL20 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-D358K

Plasmid

#91983

Purpose

Expresses FLAG-HA-AGO2 (D358K)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D358K

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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