We narrowed to 341 results for: Ky

1. ERK1C82C202

   Plasmid

   #164650

   Purpose

   Bacterial expression plasmid for His6-ERK1C82C202

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   ERK1C82C202 (MAPK1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   K71R/C144S/C178S/C183S/T202C/C271S

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9-hGeminin

   Plasmid

   #199344

   Purpose

   modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin

   Depositor

   Iain Hagan

   Article

   Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1

   Insert

   ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
   

   Use

   Tags

   cas9 c-term fused to hgemenin 1-110 fragment

   Expression

   Mammalian

   Mutation

   cas9 c-term fused to hgemenin 1-110 fragment

   Promoter

   cbh

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Δ1-60MEK1C222

   Plasmid

   #164641

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S222C/C277S/C376S; residues 1-60 at N-terminus we…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Δ1-60MEK1C218

   Plasmid

   #164643

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C218

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S218C/C277S/C376S; residues 1-60 at N-terminus we…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Δ1-60MEK1C218C222

   Plasmid

   #164645

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C218C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S218C/S222C/C277S/C376S; residues 1-60 at N-termi…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV2/9 REP-AAP-ΔCAP

   Plasmid

   #205991

   Purpose

   Rep and AAP protein expression for AAV CREATE libraries

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   AAV9 Rep and AAP proteins for CREATE libraries
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-racRNA-A

   Plasmid

   #200824

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS puro HA-hUSP27X

   Plasmid

   #225718

   Purpose

   Bacterial expression of USP27X with HA tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-U6-racRNA-B

   Plasmid

   #200825

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-U6-racRNA-C

    Plasmid

    #200826

    Purpose

    AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter

    Depositor

    Xiao Wang

    Article

    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

    Inserts

    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    

    Use

    AAV

    Tags

    C-Far, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-U6-racRNA-D

    Plasmid

    #200827

    Purpose

    AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter

    Depositor

    Xiao Wang

    Article

    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

    Inserts

    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    

    Use

    AAV

    Tags

    C-Far, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

    Plasmid

    #127343

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Flag- HA- Tandem Epitopes

    Expression

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    None

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-Neo-TetO-Cdk13_Untagged

    Plasmid

    #127344

    Purpose

    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    None

    Expression

    Mammalian

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    TetO Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cdk13_intron3_sg4_pX458

    Plasmid

    #127339

    Purpose

    sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #4

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Mouse Cdk13 Intron 3 sgRNA (Cdk13 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cdk13_intron4_sg3_pX330

    Plasmid

    #127340

    Purpose

    sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #3

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Mouse Cdk13 Intron 4 Targeting sgRNA (Cdk13 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCR8/GW_TOPO_Cdk13_Untagged

    Plasmid

    #127342

    Purpose

    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Expression

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    None

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DH10B E. coli aptI/gidB::Landing Pad

    Bacterial Strain

    #83036

    Purpose

    For site-specific chromosomal insertion of a target DNA sequence at an engineered landing pad site in the atpI/gidB locus.

    Depositor

    Cammie Lesser

    Article

    Reeves et al ACS Synth Biol. 2015 May 15;4(5):644-54.

    Bacterial Resistance

    Tetracycline

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

18. rAAV9-CAP-in-cis-Lox-AAV

    Plasmid

    #205990

    Purpose

    rAAV9 cap acceptor vector for CREATE libraries

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    rAAV9 cap acceptor vector for CREATE libraries
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Deletion between XbaI and AgeI sites

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCmini-iCAP-AAV.CAP-Mac

    Plasmid

    #200658

    Purpose

    Synthetic construct isolate AAV.CAP-Mac VP1 gene

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    Synthetic construct isolate AAV.CAP-Mac VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    7 amino acid insertion after Q588 of the AAV9 CAP…

    Promoter

    p41

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-Neo-TetO-Cdk13_Nterm_FlagHa

    Plasmid

    #127345

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    Flag- HA- Tandem Epitopes

    Expression

    Mammalian

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    TetO Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge