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pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

Plasmid

#192148

Purpose

T cell CRISPR AAV vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Use

AAV

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-GfABC1D-NLS-GFP-WPRE-synpA-L1-R2

Plasmid

#203541

Purpose

gRNA expression in astrocytes

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

NLS-GFP, 2x U6-RNA

Use

AAV

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 ElonginB, ElonginC

Plasmid

#230999

Purpose

Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Elongin B (Elob Mouse)
Elongin C (Eloc Mouse)

Expression

Bacterial

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-hKDM4A-polyA

Plasmid

#101051

Purpose

in vitro transcription (T7 promoter)

Depositor

Yi Zhang

Article

Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

Insert

KDM4A (KDM4A Human)

Tags

Flag

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

Plasmid

#218793

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

NLSf-mScarletP2A-hLuc

Use

AAV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-hKDM4A(H188A)-polyA

Plasmid

#101052

Purpose

in vitro transcription (T7 promoter)

Depositor

Yi Zhang

Article

Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

Insert

KDM4A (KDM4A Human)

Tags

Flag

Expression

Mammalian

Promoter

T7

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-sc-WDR59-SEC13

Plasmid

#237416

Purpose

Transient expression of N-terminally FLAG-tagged human WDR59-SEC13 "single chain" in the GATOR2 complex

Depositor

David Sabatini

Article

Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

Insert

WDR59-SEC13 fusion (WDR59, SEC13 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

Plasmid

#231007

Purpose

Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

E4F1 aa220-242 (E4F1 Human)

Tags

AcGFP1

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-GfABC1D-SaCas9-WPRE3-pA

Plasmid

#203540

Purpose

SaCas9 expression in astrocytes

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

SaCas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoFGFR1-D387A

Plasmid

#59778

Purpose

Expresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by light

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-D387A (FGFR1 Mustard Weed, Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pETDuet1-WT-LanCL2-C-Ter-His

Plasmid

#154186

Purpose

To express LanCL2 in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Tags

Hexahistidine

Expression

Bacterial

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

Plasmid

#192147

Purpose

T cell CRISPR lentiviral vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoFGFR1-Y766F

Plasmid

#59777

Purpose

Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-Y766F (FGFR1 Mustard Weed, Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pNeuLite PEA3 mt

Plasmid

#16248

Depositor

Mien-Chie Hung

Article

Xing et al Nat Med. 2000 Feb . 6(2):189-95.

Insert

Her-2/neu promoter (ERBB2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

PEA3 mt: PEA3 motif AGGAAG changed to AGCTCG

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pSico-syn-GFP-TVA-oG-WPRE-bGHpA

Plasmid

#231009

Purpose

Lentiviral helper virus for G-deleted rabies tracing

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Inserts

Enhanced GFP
TVA (CD320 Chicken)
optimized G

Use

Lentiviral

Promoter

human synapsin I

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMND_IgKss-V5-HA-LaccID(codon opt)-CD4 TM_pHR

Plasmid

#234048

Purpose

expresses LaccID on the T cell surface, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LaccID (codon opt_partial)-CD4TM

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pJRoC30-LaccID

Plasmid

#234652

Purpose

Expresses LaccID evolved variant in yeast

Depositor

Miguel Alcalde Galeote, Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LaccID

Tags

HRV 3C protease site-GSG linker-8xHis tag

Expression

Yeast

Mutation

V11A, Q45R, N74S, R121S, I126V, D255G, L300V

Promoter

Gal1p

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry

Plasmid

#231008

Purpose

Protein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

CDK5R1 aa283-307 (CDK5R1 Human)

Tags

AcGFP1

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual His6-TEV-CUL2, Rbx1

Plasmid

#231000

Purpose

Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Rbx1 (Rbx1 Mouse)
CUL2 (Cul2 Mouse)

Tags

6x HIS

Expression

Insect

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1-LanCL2-H264A-C-Ter-His

Plasmid

#154187

Purpose

To express LanCL2-H264A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Tags

Hexahistidine

Expression

Bacterial

Mutation

His264->Ala264

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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