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Plasmid#208185PurposeGFP positive, mScarlet-I negative controlDepositorInsertGFPmut2
UseReporterTagsExpressionBacterialMutationPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_CRFmut
Plasmid#208659PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neuronsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp R4-4 mutant
Plasmid#173491PurposeExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.DepositorInsertSfp-R44
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
-227 hTF (mutant AP-1)
Plasmid#15444DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationBoth AP-1 sites mutatedPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
DBDmut-CA-RIT-NFAT1
Plasmid#63669PurposeConstitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA bindingDepositorInsertNFAT1 (Nfatc2 Mouse)
UseRetroviralTags3x HAExpressionMammalianMutationCA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…PromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_FL MUT TET1
Plasmid#124081PurposeExpression of catalytic inactive form of full-length TET1DepositorInsertTET1 - full length, mutated (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationH1672Y, D1674APromoterEF1 alphaAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ADARcd-YTHmut
Plasmid#194431PurposeADAR1 catalytic domain E488Q fused to YTHmutDepositorInsertADARcd fused to YTHmut domain (ADAR Human)
UseTagsADARcd-YTHmut-HAExpressionMammalianMutationm6A binding region of YTH domain is deletedPromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Gmut
Plasmid#190479Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
UseTagsExpressionMammalianMutationc.1853A>GPromoterSV40Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD MUT TET1
Plasmid#124083PurposeExpression of mutated catalytic domain only form of TET1DepositorInsertTET1 - catalytic domain mutated (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationH1672Y, D1674APromoterEF1 alphaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Pro Mut E2
Plasmid#48750PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationMutated E-box2 sequence from CACGTT to GCTAGTPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBM-mut
Plasmid#187150Purposemammalian cell expression of FLAG-HUWE1 L2976A/P2977ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationL2976A/P2977APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UIM-mut
Plasmid#187148Purposemammalian cell expression of FLAG-HUWE1 A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationA1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA-mut
Plasmid#187146Purposemammalian cell expression of FLAG-HUWE1 M1328A/F1330ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationM1328A/F1330APromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6 mut C/EBP
Plasmid#61291Purposedrives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) siteDepositorInsertIL-6 promoter (Il6 Mouse)
UseLuciferaseTagsExpressionMammalianMutationMutated C/EBP binding site from ACATTGTGCAATCT to…PromoterIL-6 promoter with mutant C/EBP binding siteAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-Mt-mut
Plasmid#120830PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5 E218A "Mtase mut"
UseTagsV5ExpressionMammalianMutationE218APromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGL4-4X Mut Renilla
Plasmid#70040PurposeControl plasmid for testing 5'UTR folding elementsDepositorInsertRandom sequence matched for GC-content
UseLuciferaseTagsrenilla luciferaseExpressionMammalianMutationPromoterSV40Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
RSRF-Luc-2mut
Plasmid#31819DepositorInsertMutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
UseLuciferaseTagsExpressionMammalianMutationtwo CTATATTTAG RSRF sites mutated to CGATATTTCGPromotermutated MEF2-dependent (-307 to -242 of Nur77/NR4…Available sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCV LDTS-LIRmut
Plasmid#189001Purposelipophagy-inducing adaptor protein, negative controlDepositorInsertLDTS-LIRmut
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 _3xHA-Ago2_RNAmut
Plasmid#73539PurposeInducible lentiviral expression of Ago2_RNAmutDepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationSmall RNA binding mutant PAZ domain Y311A and F31…PromoterTRE promoter, Tet ONAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pCaf-mCherry
Plasmid#192860PurposepMUT2 based vector for sensing caffeine in E. coli Nissle 1917DepositorInsertNanobody-CadC fusion protein and cognate promoter inducible by caffeine
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only